554 Properties of an Antityphoid Serum obtained from the Goat. 



Conclusions. 



1. The intravenous injection of the goat with the toxic cell juices of the 

 B. typhosus (obtained under the conditions described) in small and carefully 

 regulated doses resulted in the production of an antiendotoxin. 



2. The antiendotoxic value, as so far tested, reached a point at which 

 1/50 c.c. of the serum neutralised 30 lethal doses of the toxic typhoid cell 

 juice. This action was not demonstrable in 3 c.c. of normal goat's serum, 

 and was obtained after about four months' treatment of the goat. The 

 results, after a more rapid method of immunisation, are better qua goat and 

 rabbit than those obtained by Dr. Besredka in the course of two years with 

 dead and living bacilli qua horse and guinea-pig. 



3. The serum was also agglutinative for the B. typhosus, the titrate rising 

 to 1/1,000,000. 



4. The serum was also bacteriolytic, 1/10000 c.c. neutralising 10 lethal 

 doses of the B. typhosus. 



5. The serum did not give a precipitin reaction with typhoid cell juices. 



6. The serum whilst neutralising the typhoid did not neutralise the 

 cholera endotoxin. 



My next step will be to test in how far it is possible to obtain analogous 

 results in the horse. 



Analogous results have been obtained indicating the production of an anti- 

 body for the endotoxin of the cholera organism. 



I am greatly indebted to Mr. E. T. Thompson for invaluable aid as well 

 as for an important modification which has rendered the grinding process 

 void of danger. 



