1912.] 



Islets of Langerhans to the Pancreatic Acini. 



81 



according to the number of days the animals were allowed to survive 

 operation. 



Experiment 1. — Young female. Weight, 5'1 kgrru. Rather fat. Morphine, ether, 

 flemoval of the pancreas except for a segment at the duodenal end, measuring 1*5 x 1*5 cm., 

 which is tied off from the intestine and the rest of the organ with silk. With its blood 

 supply intact it is transplanted through a slit in the oblique muscles to a space outside 

 the muscles. Good recovery from operation, but the animal, though it does not look ill, 

 remains quiet for the next three days. Urine for the second 24 hours following opera- 

 tion 250 c.c. It contains 3 per cent, sugar. On the morning of the fourth day the 

 animal looks ill, has lost fat, and a fluctuating mass is felt at the site of the graft. 

 Killed by bleeding under ether. Examination shows a thin, yellowish fluid surrounding 

 the graft, which appears white and opaque. No pancreatic remains found in the abdomen. 



Microscopic ^Examination. — Superficial infection of the graft. Acinous cells highly 

 charged with zymogen. Apparent slight increase of duct tissue. The islets appear 

 normal in size and shape. The B cells are for the most part small, and very few of the 

 typical slate blue granules are seen. The A cells stand out darkly stained and 

 prominent. 



Experiment 2. — Adult female. Weight, 8 kgrm. Good condition. Morphine, ether. 

 Removal of the pancreas except for a piece 2x2 cm. tied off with silk at the duodenal 

 end. Transplanted with its blood supply outside the oblique muscles. Considerable 

 handling of the transplant. Good recovery from ether. Twelve hours' specimen of 

 urine on night of third day contained 3'35 per cent, of sugar. 



7th day. — Animal thin but seems lively and eats and drinks well. Urine for 

 17 hours (550 c.c.) contains 3'95 per cent, of sugar. 



8th day. — Wound puffy and graft swollen. 



10th day.— Site of graft looks better. Animal lively. Sugar, 1*9 per cent, (single 

 specimen). 



13th day. — 24 hours' urine contains 4 - 5 per cent, of sugar. The graft looks normal, 

 and the animal seems lively and well. 



16th day. — Killed by bleeding under ether. 



Examination shows that the site of the graft is clean. A moderate amount of scar 

 tissue surrounds the graft, which appears about the same extent as at operation, but 

 considerably thicker ; white, opaque, and very firm on section. No pancreatic remains 

 found in abdomen. 



Microscopic Examination. — The acinous cells are highly charged with zymogen. They 

 appear slightly shrunken, though there is no evident destruction. There is a great 

 relative, and probably absolute, increase of small ducts and centroacinous cells. No 

 mitotic figures are found. The islets appear considerably altered. The B cells stain 

 poorly, showing few slate blue granules. They contain some red and some uncoloured 

 granular material, and are generally shrunken. 



Experiment 3. — Adult female. Weight, 9'6 kgrm. Good condition. Morphine, 

 ether. Removal of the pancreas except for a piece 2x2 cm. tied off with silk at 

 duodenal end and left in place with blood supply intact. Slow recovery from ether, and 

 animal seems very quiet on the day following. 



3rd day. — Animal lively and well. 



7th day. — Animal eats and drinks well. Urine contains no sugar. 

 10th day. — Weight, 9 kgrm. Animal seems well. 



20th day. — Weight, 8"75 kgrm. Animal seems well. Overnight urine contains 

 2 per cent, sugar (estimated). 



26th day. — Animal in good condition. Killed by bleeding under ether. 



