82 



Dr. J. Homans. The Relation of the [Oct. 1 , 



Examination. — There is a little clear fluid about one end of the graft, which is thick, 

 white, and opaque. The other end, or about one-half of the original fragment, is thin, 

 grey, and scar-like, but shows the normal markings. No other pancreatic remains found 

 in the abdomen. 



Microscopic Examination. — Sections from the swollen opaque end differ in no essential 

 particular from those of Experiment 2 (16-day graft) except that the islets are rather 

 better preserved. The B cells are very few in number, which takes away from the islet 

 its characteristic appearance (fig. 6), but it is easy to identify. Sections from the 

 atrophied end present appearances shown in fig. 5. The acinous cells still contain 

 zymogen granules and stain characteristically, but they are shrunken and gathered into 

 small compressed groups. There is an obvious relative increase of duct and centro- 

 acinous tissue. A few islets can be found, but they take almost none of the bluish stain 

 characteristic of B cells, and as they thus resemble duct cells (compare figs. 5 and 6) they 

 are not easy to identify. They are not, however, increased in size, nor is there any 

 evidence of a change of acini into islet tissue. My impression is that the islets are 

 undergoing destruction by the scar tissue. 



Experiment 4. — Adult female. Weight, 6 kgrm. Rather thin. Morphine, ether. 

 Duodenal end of gland tied off as usual and rest of pancreas removed. Transplantation 

 of fragment 2x2 cm. with its blood supply intact outside the oblique muscles. Good 

 recovery from ether. Although it has lost some weight the animal remains lively and 

 well until just before it is killed. 



8th day. — Urine contains no sugar. Graft plainly felt through skin, which moves 

 freely over it. 



29th day. — Animal seems well. Overnight urine (250 c.c.) contains 2'7 per cent, sugar. 



33rd day. — The animal appears ill and has not eaten during the last 24 hours. Killed 

 by bleeding under ether. 



Examination. — Peritonitis following perforation from fuecal impaction (undigested 

 meat). The graft is shrunken, grey, and scar-like, perhaps two-thirds its original size. 

 A firm elastic cyst is embedded in it. The lobulation is still evident. 



Microscopic Examination. — The whole graft is invaded by scar tissue. The acini are 

 broken up into small groups. The acinous cells are generally well enough preserved to 

 be identified, and contain many zymogen granules. On the whole the general colour of 

 the protoplasm is less evident than usual, and only the group arrangement and the 

 presence of typical zymogen granules allow the acinous cells to be distinguished. The 

 duct and centroacinous cells seem to be increased in number, and when found in groups 

 resemble islets. A great many cells scattered through the specimen have the appearance 

 of islet cells, but on looking through 30-40 sections I have found no complete typical 

 islets. 



Duct Ligation. 



Experiment 5. — Adult female. Weight, 7 - 3 kgrm. In good condition. Morphine, 

 ether. Ligation of ducts. Good recovery from ether. 

 4th day. — 24 hours' specimen of urine contains no sugar. 

 11th day.— Weight, 6"25 kgrm. 

 18th day. — Weight, 7 kgrm. 

 28th day.— Weight, 7"25 kgrm. 



35th day. — No sugar in overnight urine. Weight, 7"75 kgrm. Animal seems fat and 

 well. Killed by bleeding under ether. 



Examination. — The pancreas is shrunken to two-thirds its original length. The whole 

 splenic end is much narrowed and a little thickened. It appears pale and scar-like. 

 The duodenal end is much the same. The region of the duct outlets for about 3 cm. is 

 broad, thick, white, and opaque, resembling exactly the 16-day (Experiment 2) and 



