270 



Sir D. Bruce and others. Trypanosomes in [Jan. 13, 



When an animal is shot in this fly-country by a member of the Com- 

 mission a small quantity of the blood is taken in a bottle containing citrate 

 of soda solution for inoculation purposes, and a thick and thin film of the 

 blood spread on glass slides for microscopical examination. The blood is 

 then sent to a point on one of the main paths, where a motor-cyclist is 

 waiting to carry it to the camp. "When the blood arrives it is at once 

 injected into a goat, a monkey, and a dog. 



Wild Animals Living in a Fly-area as Hosts or Eeservoirs of 

 Trypanosome Disease. 



The following table represents the result of the examination of 180 speci- 

 mens of wild game or other animals shot in this fly-area. In one column is 

 given the number of hours between the taking of the blood and its 

 inoculation, in others the result of the microscopical examination of the 

 thick and thin film, and of the inoculation of the blood. A plus sign means 

 that trypanosomes are present, a minus sign that they are absent, and 

 a blank space that a microscopical specimen was not available for examina- 

 tion, or an animal for inoculation, as the case may be. 



Table I. — List of Wild Animals living in a Fly-area whose Blood has been 

 Examined for Trypanosomes. 



Date. 



Expt. 

 No. 



Animal. 



Age of 

 blood, in 

 hours. 



By microscopical 

 examination. 



By inoculation. 



Thick. 



Thin. 



Goat. 



Monkey. 



Dog. 



1912. 



















Jan. 20 .... 



32 



Hartebeeste ... 















„ 20 ... 



33 

















„ 20 ... 



34 

















„ 22 ... 



40 



;; ;;: 















„ 22 ... 



41 



Eland" 













+ 



„ 22 ... 



41 











+ 







„ 23 ... 



62 



Sable 















„ 23 ... 



63 

















„ 26 ... 



95 



Eland 















Feb. 6 ... 



159 



Warthog 















„ 6 



160 



Sable 















„ 18 ... 



246 

















„ 23 ... 



283 

















„ 28 ... 



288 



Lion 















May 19 ... 



615 



Oribi 



3 













„ 19 ... 



616 



Waterbuck . . . 



17 





+ 









„ 25 ... 



583 



Duiker 















„ 25 ... 



584 



Warthog 















„ 26 ... 



589 

















„ 26 ... 



I 591 









+ 









„ 26 ... 



! 593 



Duiker «.. 















