302 



Messrs. W. Cramer and J. Lochhead. [Jan. 16, 



Conclusions. 



1. The five human strains of this trypanosome, isolated from four natives 

 in Nyasaland and one European in Portuguese East Africa, belong to the 

 same species. 



2. This species is T. rhodesiense (Stephens and Eantham). 



3. Evidence is accumulating that T. rhodesiense and T. brucei (Plimmer and 

 Bradford) are identical. 



Contributions to the Biochemistry of Growth* — The Gly cog en- 

 content of the Liver of Rats bearing Malignant Neiv Growths. 



By W. Cramer and Jas. Lochhead. 



(Communicated by Prof. E. A. Schafer, F.R.S. Received January 16, — 

 Read February 20, 1913.) ■ 



(From the Physiology Department, University of Edinburgh, and the Imperial Cancer 



Research Fund, London.) 



In previous papers by one of us (1, 2) observations on the gaseous 

 metabolism and on the nitrogen metabolism of tumour-bearing rats have 

 been recorded. The present paper contains observations on the carbo- 

 hydrate metabolism of tumour- bearing rats. The tumour-strain employed 

 in our previous work was also used for these experiments; it is a spindle- 

 celled sarcoma (J.R.S. of the Imperial Cancer Research Fund) which has a 

 rapid growth. This tumour does not contain any glycogen. A large 

 number of glycogen estimations were carried out with the liver of normal 

 and of tumour-bearing animals. The glycogen estimations were made by 

 Pfluger's method ; the glucose obtained by the hydrolysis of glycogen was 

 estimated in the first part of the work gravimetrically according to the 

 technique used by Pfluger; later on, Bertrand's method of titration was 

 employed. It is necessary to bear in mind that only 4 to 6 grm. of liver 

 tissue are available for analysis in these estimations, so that with a glycogen 

 percentage below 01 to 0"2 per cent, the absolute amount of glycogen 

 present in the whole liver of a rat is so small that it cannot be determined 



* This research is in continuation of papers in 'Roy. Soc. Proc.,' 1908, B, vol. 80, 

 p. 263 ; 1910, vol. 82, p. 307 ; ibid., p. 316. 



