362 Messrs. Bainbridge, Collins, and Menzies. [Mar. 27., 



Table II. — Effect of Poisoning the Tubules with Corrosive Sublimate. The 

 perfusing fluid was in all cases hypotonic Einger's solution. 



1 



Exp. 



Concentration of urine. 



Concentration of 



Histological result. 



Normal kidneys. 



Poisoned kidneys. 



perfusing fluid. 



1 

 2 



3 



per cent. 

 -33, -29, -40 

 -48, 0-43 



-30 

 '47, -40 



per cent. 

 0-53, 52 

 0-60 

 40 

 0-51 



0-53 per cent. NaCl~| 

 0-58 „ „ 1 



0-57 „ „ J 



Glomeruli intact, 

 tubules all show Hg. 



Different figures represent separate samples of urine. 



(6) The kidneys were first perfused with oxygenated Einger's solution 

 simultaneously by the aorta and renal portal veins. Then the glomeruli 

 were perfused with boiled Einger's solution in order to kill them, while the 

 tubules were still receiving by the veins an adequate supply of oxygen. 

 A typical experiment is shown in the following protocol : — 



Protocol II. — Pithed Male Frog. Cannulae in left aorta, anterior abdominal 

 vein, inferior vena cava and ureters. Mesenteric artery and right aorta 

 ligatured. Hind legs tied off. Simultaneous arterial and venous 

 perfusion. Arterial pressure 24 cm., venous 12 cm. Molecular concen- 

 tration of Einger's solution (refractometer) = - 55 per cent. NaCl. 

 Einger's solution contained 0*5 per cent. NaCl, 0'02 per cent. KC1, 

 - 03 per cent CaCU in distilled water. 



Time. 



Escape from 



Urine. 



Concentration of urine. 



vena cava. 







c.c. 







11.47-12.4 



31 



n An i o in f B.. K. - 1 C.C. 



11.40-12.10 | L E . 15 n 



-40 per cent. NaCl 

 0-46 „ 



12.4-12.28 



51 







12.28-12.47 



37 



i2.io-i 2 .48{j| ; o;; 5 5 » 



-33 „ 

 -34 „ 



12.47-1.17 



33 



12.48-2.15, both kidneys -1 c.c. 



36 „ 



1.17-1.47 



■ 27 





1.47-2.17 



23 







2.17-2.47 



25 







12.50 onwards. Glomeruli perfused with boiled Einger's solution. 



It will be noticed that the cutting off of the oxygen supply to the 

 glomeruli lessened the rate of arterial perfusion and also the amount of urine 

 formed. The general result of a number of such experiments is shown in 

 the following table : — 



