382 Major W. B. Fry and Captain H. S. Eanken. [Dec. 23, 



granule is forcibly ejected by energetic movements of the trypanosome it is 

 flung out into the free fluid to some distance ; this is the most usual method. 



Some trypanosomes seem to be acutely poisoned by the antimony, and 

 death and complete dissolution occur very rapidly. This is more frequently 

 seen when the preparation is made 5 to 7 minutes after injection of antimony. 

 The trypanosome becomes anchored, its lashing movement slows down and 

 comes to a standstill, the body swells and becomes bloated, losing its 

 characteristic form. In this condition it is devoid of energy and can no 

 longer forcibly extrude granules, but the latter have not suffered so severely 

 and may still show an excited dancing movement inside the degenerate 

 trypanosome body, which appears to give way before this activity, and the 

 granule may ultimately work its way clear of the degenerate protoplasm and 

 inelastic covering of the now dead, or dying, trypanosome. 



In other instances the trypanosome does not die so rapidly and the 

 granules, after continuing this dancing movement inside it for some time, 

 gradually come to rest before the trypanosome has reached so advanced a 

 state of degeneration as to permit a dancing granule to escape by its own 

 efforts. The degeneration of the trypanosome continues till it has lost outline 

 and refractility and can only be recognised as an ill-defined " ghost," 

 enveloping the granules which are held in position — more or less in the 

 original long axis of the trypanosome — by this viscid protoplasm. This is 

 the last stage that can be seen in a dark-ground preparation where the objects 

 are at rest. In the living subject, however, it is probable that this degenerate 

 protoplasm would not be allowed to remain at rest, but would be broken up 

 by the active currents and eddies and the granules would thus be set free. 



Thus there are probably three methods by which a grannie may be liberated 

 from the parent trypanosome : — 



(1) By the activity of the trypanosome — forcible extrusion. 



(2) By the active movement of the granule in a rapidly degenerating 

 trypanosome. 



(3) By outside agencies, eddies, currents, etc., which may break up a 

 degenerate trypanosome when the contained granules are unable to effect their 

 escape. 



In some cases extrusion occurs rapidly. A trypanosome has been seen to 

 extrude two large granules and immediately afterwards break up — the whole 

 process being complete in 20 minutes. 



In the early preparations (3 minutes) the exaggerated motility is a 

 prominent feature and forcible extrusion is most commonly seen ; in the 

 later films (7 minutes) the antimony has had longer time to act and the 



