392 Major W, B. Fry and Captain H. S. Kanken. [Dec. 23, 



Fig. 3. — A similar elongated granule is seen completely separated from the nucleus. 

 There is a faint indication of a halo surrounding it. 

 4. — A large elongated granule is seen between the macro- and micronuclei, lying 

 close to the periplast. 



„ 5. — Several granules are present ; one is just being detached from the macronucleus. 



„ 6. — Two granules are seen on the point of escaping from the trypanosome ; the 

 larger looks as if it is nearly extruded. 



„ 7. — A recently extruded granule is seen near the trypanosome. The macronucleus 

 shows two deeply stained points— probably granules becoming differentiated 

 in its substance before being budded off. 



„ 8. — Two granules, lying between the macro- and micronuclei, are each seen to be 

 surrounded by a well-defined clear hyaline area. Two others are almost com- 

 pletely separated from the macronucleus. 



„ 9. — Free granule ; no differentiation. 



,, 10. — Free granule, larger, and with a faint rim of cytoplasm. 



., 11. — King-shaped nucleus with micronucleus coming off ; definitely more protoplasm 



than the previous form. 

 ,, 12. — Early form with macro- and micronucleus and pale blue-staining cytoplasm. 

 „ 13. — Similar form, larger. 



„ 14. — The nucleus has divided in this specimen, while there is only one micronucleus 

 seen. 



15. — Both macro- and micronuclei are divided. 



16. — Micronuclei only have divided ; macronucleus in process of division. 



„ 17-26. — All are similar forms. They vary in shape and correspond closely with the 



forms seen by vital staining of emulsions of internal organs. 

 ,, 20 and 22. — Show division of the micronuclei. 

 „ 21 and 22. — Show the third chromatin body described. 

 ,, 25. — Shows division of macro- and micronuclei. 



„ 27. — A single form, with macro- and micronucleus, and a very long flagellum. 



Plate 11. 



Figs. 1-12. — The specimens were found in smear preparations from the liver and 

 kidney from rats infected with T. rhodesiense. They show dividing forms in various 

 stages. 



Figs. 13-20. — Blood from liver of rat infected with T. rhodesiense. ■ Immature trypano- 

 somes are shown gradually merging into adult forms. 



Fig. 1. — Early stage of division. There are already two micronuclei, but the macro- 

 nucleus is just beginning to divide. 



„ 2. — This shows similar division to fig. 1, but a little further advanced. The macro- 

 nucleus is now in the stage of mitosis. 



„ 3. — Complete separation of macro- and micronuclei, but the flagella are not yet 

 separated. 



„ 4. — Two form with nuclei and flagella completely divided ; one flagellum is much 

 longer than the other and lies round the margin of the body. 



„ 5. — Two form beginning to divide into two independent bodies which are identical 

 with the early immature forms shown in figs. 13-15. 



„ 6. — Two form. The nuclei have moved to some distance from each other. A thick 

 f;in is seen in the shorter of the two flagella. 



