1913.] 



Studies on Enzyme Action. 



597 



The retarding effect of oleic acid is shown more clearly in the following 

 table and diagram representing the results obtained in experiments in 

 which the rate of change was contrasted in presence of various proportions 

 of one or other or both products of change : — 



Table showing Percentage of Change effected by 0'5 grm. of Enzyme acting 

 on 5 grm. of Olive Oil and 2 c.c. "Water at 30° C. (0 = 1 molecular 

 proportion of Oleic Acid ; G do. Glycerol.) 



Time in 



Oil alone 



Oil + 30 



Oil + 6 



Oil + G 



OU + 2G 



Oil + 3 + G 



hours 



Graph A 



Graph B 



Graph C 



Graph D 



Graph E 



Graph F 



1 



17 -9 



9-5 



5-6 



15 -7 



13 -0 



6-6 



2 



26 -3 



16 -1 



8-9 



26 -1 



20 -1 



11 -3 



4 



35 -4 



27 -0 



16 9 



36 -4 



33 1 



18 -5 



6 



44 -9 



36 -4 



21 -2 



46 -1 



39 6 



21 -8 



8 



52 -3 



44 -2 



26 -3 



51 "6 



44 -8 



26 -1 



16 



62 -8 



57 9 



38 -2 



61 -9 



57 2 



36 -8 



25 



70 -1 



65 2 



44-5 



69 -8 



65 -2 



44-5 



50 



78-9 



(48 hours) 



69 -7 



47 -7 



78 3 



72 2 



51 -1 



O IO 20 30 40 u 50 



Hours. 



We are inclined to attribute the slight effect glycerol produces to altei'a- 

 tions in the osmotic conditions affecting the enzyme-oil interface. On the 



