﻿88 
  Dr. 
  W. 
  B. 
  Bottomley. 
  The 
  Effect 
  of 
  Nitrogen-fixing 
  

  

  a 
  greenhouse 
  as 
  before. 
  The 
  solutions 
  were 
  changed 
  twice 
  weekly 
  and 
  the 
  

   plants 
  counted 
  once 
  weekly, 
  and 
  when 
  they 
  had 
  filled 
  their 
  dishes 
  in 
  some 
  of 
  

   the 
  series, 
  as 
  they 
  had 
  at 
  the 
  end 
  of 
  the 
  second 
  week 
  in 
  Series 
  V 
  and 
  VI, 
  the 
  

   number 
  throughout 
  the 
  set 
  was 
  halved 
  at 
  the 
  weekly 
  counting, 
  only 
  one-half 
  

   of 
  each 
  dish 
  being 
  retained. 
  This 
  was 
  repeated 
  each 
  week 
  throughout 
  the 
  

   experiment, 
  which 
  lasted 
  six 
  weeks, 
  the 
  plants 
  being 
  even 
  quartered 
  when 
  

   necessary. 
  Table 
  II 
  shows 
  the 
  figures 
  obtained, 
  which 
  represent 
  the 
  total 
  

   numbers 
  of 
  plants 
  arising 
  from 
  the 
  original 
  ten 
  in 
  each 
  dish 
  : 
  — 
  

  

  Table 
  II. 
  

  

  Series. 
  

  

  

  1st 
  

  

  2nd 
  

  

  .11 
  

   ora 
  

  

  4tli 
  

  

  oto 
  

  

  otu 
  

  

  No. 
  

  

  wees.. 
  

  

  week. 
  

  

  week. 
  

  

  week. 
  

  

  week. 
  

  

  weefe. 
  

  

  I. 
  

  

  [ 
  

  

  1 
  

  

  29 
  

  

  84 
  

  

  256 
  

  

  352 
  

  

  704 
  

  

  1,472 
  

  

  

  2 
  

  

  33 
  

  

  92 
  

  

  224 
  

  

  384 
  

  

  832 
  

  

  2,176 
  

  

  Detmer's 
  Solution 
  ... 
  

  

  < 
  

   1 
  

  

  3 
  

  

  32 
  

  

  96 
  

  

  208 
  

  

  384 
  

  

  704 
  

  

  1,536 
  

  

  

  4 
  

  

  34 
  

  

  96 
  

  

  176 
  

  

  416 
  

  

  1,024 
  

  

  2,112 
  

  

  

  I 
  

  

  X 
  

  

  o 
  

  

  oo 
  

  

  oo 
  

  

  

  Oat 
  

  

  / 
  Oo 
  

  

  1 
  7Q9 
  

  

  Mean 
  

  

  

  

  30 
  *2 
  

  

  91 
  '2 
  

  

  214 
  - 
  4 
  

  

  384 
  - 
  

  

  806 
  - 
  4 
  

  

  1,817 
  '6 
  

  

  II. 
  

  

  

  

  

  

  

  

  

  

  Detmer's 
  Solution 
  

  

  f 
  

  

  6 
  

   7 
  

  

  44 
  

   46 
  

  

  212 
  

   242 
  

  

  900 
  

   904 
  

  

  3,008 
  

   3,304 
  

  

  16,896 
  

   18,248 
  

  

  109,824 
  

   104,416 
  

  

  + 
  

  

  Crude 
  Nucleic 
  Acid 
  

   Derivatives 
  

  

  1 
  

  

  1 
  

  

  8 
  

   9 
  

  

  39 
  

   38 
  

  

  238 
  

   220 
  

  

  836 
  

   832 
  

  

  3,160 
  

   3,264 
  

  

  16,568 
  

   16,128 
  

  

  84,528 
  

   92,400 
  

  

  I 
  

  

  

  4,7 
  

  

  244 
  

  

  864 
  

  

  

  

  

  

  

  

  42-8 
  

  

  231 
  -2 
  

  

  867 
  -2 
  

  

  3,123 
  -2 
  

  

  16,537 
  -6 
  

  

  96,921 
  -6 
  

  

  III. 
  

  

  

  

  

  

  

  

  

  

  

  r 
  

   i 
  

  

  11 
  

  

  40 
  

  

  184 
  

  

  560 
  

  

  1,792 
  

  

  7,936 
  

  

  33,024 
  

  

  Detmer's 
  Solution 
  

  

  12 
  

  

  41 
  

  

  196 
  

  

  640 
  

  

  1,984 
  

  

  7,168 
  

  

  30,208 
  

  

  + 
  

  

  i 
  

  

  ^ 
  

  

  13 
  

  

  41 
  

  

  160 
  

  

  496 
  

  

  1,536 
  

  

  6,912 
  

  

  27,904 
  

  

  Dinucleotide 
  

  

  t 
  

  

  14 
  

  

  36 
  

  

  172 
  

  

  560 
  

  

  1,728 
  

  

  8,448 
  

  

  35,584 
  

  

  

  i 
  

  

  15 
  

  

  38 
  

  

  184 
  

  

  608 
  

  

  1,664 
  

  

  6,912 
  

  

  31,232 
  

  

  

  

  

  39 
  2 
  

  

  179-2 
  

  

  572 
  -8 
  

  

  1,740 
  -8 
  

  

  7,475 
  -2 
  

  

  31,590 
  -4 
  

  

  IV. 
  

  

  

  

  

  

  

  

  

  

  

  r 
  

   i 
  

  

  16 
  

  

  40 
  

  

  168 
  

  

  592 
  

  

  2,048 
  

  

  12,288 
  

  

  69,376 
  

  

  Detmer's 
  Solution 
  

  

  17 
  

  

  40 
  

  

  232 
  

  

  816 
  

  

  2,752 
  

  

  16,128 
  

  

  81,920 
  

  

  + 
  

  

  i 
  

  

  18 
  

  

  45 
  

  

  208 
  

  

  784 
  

  

  3,072 
  

  

  18,432 
  

  

  91,136 
  

  

  Autoclaved 
  

  

  19 
  

  

  41 
  

  

  240 
  

  

  736 
  

  

  2,496 
  

  

  14,592 
  

  

  73,728 
  

  

  Azotobacter 
  

  

  i 
  

  

  20 
  

  

  42 
  

  

  212 
  

  

  784 
  

  

  2,944 
  

  

  17,664 
  

  

  84,736 
  

  

  

  

  

  41-6 
  

  

  212 
  -0 
  

  

  742 
  -4 
  

  

  2,662 
  -4 
  

  

  15,820 
  -8 
  

  

  80,179 
  -2 
  

  

  