﻿Dr. 
  H. 
  H. 
  Dale 
  

  

  138 
  

  

  Fig. 
  4 
  shows 
  a 
  record 
  of 
  the 
  reaction 
  taken 
  on 
  a 
  faster 
  moving 
  surface. 
  

   The 
  addition 
  to 
  the 
  bath 
  of 
  Ringer's 
  solution 
  of 
  a 
  small 
  dose 
  of 
  the 
  antigen 
  

  

  Kr4 
  

  

  t 
  

  

  k 
  

  

  I 
  I 
  I 
  I 
  f 
  I 
  I 
  ■ 
  I 
  I 
  ■ 
  ' 
  I 
  I 
  1 
  1 
  ' 
  

   10" 
  

  

  Fig. 
  3. 
  Fig. 
  4. 
  

  

  Fig. 
  3 
  (from 
  ' 
  Journ. 
  Pharmacol, 
  and 
  Exp. 
  Therap.,' 
  vol. 
  4, 
  p. 
  177). 
  

   Fig. 
  4 
  (from 
  'Journ. 
  Pharmacol, 
  and 
  Exp. 
  Therap.,' 
  vo!. 
  4, 
  p. 
  178). 
  

  

  to 
  which 
  the 
  plain 
  muscle 
  is 
  sensitive, 
  is 
  signalled 
  by 
  a 
  small 
  displacement 
  

   of 
  the 
  recording 
  lever. 
  The 
  response 
  begins 
  within 
  10 
  seconds, 
  with 
  no 
  

   greater 
  delay 
  than 
  that 
  shown 
  by 
  the 
  response 
  to 
  many 
  drugs 
  similarly 
  

   applied. 
  It 
  is 
  difficult 
  to 
  suppose 
  that 
  this 
  interval 
  suffices 
  for 
  the 
  elabora- 
  

   tion 
  of 
  a 
  poison 
  from 
  the 
  antigen 
  and 
  the 
  body 
  fluids, 
  whether 
  intracellular 
  

   or 
  extracellular. 
  It 
  suggests 
  rather 
  that 
  the 
  antigen 
  acts 
  as 
  a 
  direct 
  poison 
  

   on 
  the 
  specifically 
  sensitive 
  cells. 
  

  

  It 
  has 
  been 
  suggested 
  that, 
  though 
  a 
  cellular 
  location 
  of 
  the 
  antibody 
  may 
  

   account 
  for 
  the 
  phenomena 
  of 
  active 
  anaphylaxis, 
  those 
  of 
  passive 
  anaphylaxis 
  

   must 
  be 
  due 
  to 
  an 
  interaction 
  between 
  antigen 
  and 
  antibody 
  in 
  the 
  circula- 
  

   tion. 
  The 
  experiments 
  of 
  Weil, 
  to 
  which 
  I 
  have 
  already 
  referred, 
  served 
  to 
  

   make 
  it 
  clear 
  that 
  passive 
  anaphylaxis 
  only 
  develops 
  as 
  the 
  transferred 
  anti- 
  

   body 
  leaves 
  the 
  circulation 
  for 
  the 
  tissue 
  cells. 
  Fig. 
  5, 
  from 
  an 
  experiment 
  on 
  

   a 
  passively 
  anaphylactic 
  guinea-pig, 
  shows 
  that 
  in 
  this 
  condition 
  also 
  the 
  

   bloodless 
  plain 
  muscle 
  is 
  specifically 
  sensitive. 
  

  

  The 
  records 
  in 
  figs. 
  6 
  and 
  7 
  are 
  from 
  a 
  single 
  experiment 
  and 
  show 
  that 
  

   the 
  sensitive 
  plain 
  muscle, 
  after 
  exposure 
  to 
  an 
  effective 
  dose 
  of 
  the 
  antigen 
  

   (A), 
  loses 
  its 
  sensitiveness 
  to 
  a 
  further 
  dose 
  (B), 
  but 
  that 
  it 
  can 
  again 
  be 
  

   rendered 
  sensitive 
  by 
  exposure 
  in 
  vitro 
  for 
  some 
  hours 
  to 
  serum 
  from 
  sensitive 
  

   guinea-pigs. 
  When 
  this 
  is 
  washed 
  away 
  completely 
  by 
  numerous 
  changes 
  of 
  

   Ringer's 
  solution 
  the 
  plain 
  muscle 
  again 
  responds 
  to 
  the 
  antigen 
  (E), 
  but 
  is 
  

  

  