﻿208 
  

  

  Prof. 
  B. 
  Moore 
  and 
  Mr. 
  T. 
  A. 
  Webster. 
  

  

  No. 
  6. 
  — 
  Tap-water, 
  algae, 
  0*05 
  per 
  cent, 
  of 
  Na 
  2 
  HP0 
  4 
  screwed 
  down 
  glass 
  

   lid, 
  kept 
  in 
  light. 
  Nitrogen 
  = 
  TO 
  mgrm. 
  

  

  No. 
  7. 
  — 
  Tap-water, 
  algae, 
  0*05 
  per 
  cent, 
  of 
  Na 
  2 
  HP0 
  4 
  , 
  - 
  05 
  per 
  cent, 
  of 
  

   Na 
  2 
  N0 
  2 
  , 
  lawn 
  cover 
  only, 
  kept 
  in 
  light. 
  Nitrogen 
  = 
  12'3 
  mgrm. 
  

  

  No. 
  8. 
  — 
  Tap-water, 
  algae, 
  0-05 
  per 
  cent, 
  of 
  Na 
  2 
  HP0 
  4 
  , 
  0-05 
  per 
  cent, 
  of 
  

   NaN02, 
  screwed 
  down 
  glass 
  lid, 
  kept 
  in 
  light. 
  Nitrogen 
  = 
  - 
  7 
  mgrm. 
  

  

  No. 
  9. 
  — 
  Tap-water, 
  algae, 
  0'05 
  per 
  cent. 
  Na 
  2 
  HP0 
  4 
  , 
  - 
  05 
  per 
  cent. 
  NaN0 
  2 
  , 
  

   formaldehyde 
  vapour 
  at 
  high 
  dilution 
  supplied 
  from 
  side 
  tube 
  as 
  described 
  

   below, 
  glass 
  lid 
  screwed 
  down, 
  kept 
  in 
  light. 
  Nitrogen 
  = 
  3 
  8 
  mgrm. 
  

  

  No. 
  10. 
  — 
  Tap-water, 
  algae, 
  0'05 
  per 
  cent. 
  Na 
  2 
  HP0 
  4 
  , 
  no 
  NaN0 
  2 
  , 
  formal- 
  

   dehyde 
  vapour, 
  glass 
  lid 
  screwed 
  down, 
  kept 
  in 
  light. 
  Nitrogen 
  = 
  1*0 
  mgrm. 
  

  

  No. 
  11. 
  — 
  Tap-water, 
  algae, 
  - 
  05 
  per 
  cent. 
  Na 
  2 
  HP0 
  4 
  , 
  - 
  05 
  per 
  cent. 
  NaN0 
  2 
  , 
  

   dilute 
  methylic 
  alcohol 
  vapour 
  supplied 
  from 
  side 
  tube, 
  glass 
  lid 
  screwed 
  

   down, 
  kept 
  in 
  light. 
  Nitrogen 
  = 
  7'4 
  mgrm. 
  

  

  No. 
  12. 
  — 
  Tap-water, 
  algae, 
  0-05 
  per 
  cent. 
  Na 
  2 
  HP0 
  4 
  , 
  no 
  NaNO 
  a 
  , 
  dilute 
  

   methylic 
  alcohol 
  vapour 
  from 
  side 
  tube, 
  glass 
  lid 
  screwed 
  down, 
  kept 
  in 
  light. 
  

   Nitrogen 
  = 
  1*1 
  mgrm. 
  

  

  Notes 
  on 
  Growth. 
  — 
  These 
  were 
  taken 
  at 
  frequent 
  intervals 
  and 
  were 
  con- 
  

   cordant 
  throughout, 
  so 
  as 
  to 
  save 
  space 
  only 
  two 
  samples 
  will 
  be 
  given. 
  It 
  may 
  

   be 
  noted 
  that 
  in 
  the 
  course 
  of 
  growth 
  the 
  experiment 
  was 
  demonstrated 
  to 
  

   many 
  scientific 
  friends, 
  and 
  exhibits 
  made 
  at 
  meetings 
  of 
  societies, 
  such 
  as 
  the 
  

   Bio-Chemical 
  and 
  Royal 
  Microscopical 
  Societies, 
  and 
  it 
  was 
  admitted 
  that 
  

   the 
  differences 
  in 
  growth 
  existed 
  ; 
  this 
  is 
  confirmed 
  by 
  the 
  nitrogen 
  deter- 
  

   minations 
  now 
  given 
  at 
  the 
  conclusion 
  of 
  the 
  experiment. 
  

  

  July 
  1, 
  1918. 
  — 
  No. 
  1, 
  no 
  growth 
  ; 
  No. 
  2, 
  slight 
  green 
  growth 
  ; 
  Nos. 
  3 
  and 
  4, 
  

   no 
  growth 
  ; 
  No. 
  5, 
  growth 
  in 
  clumps 
  ; 
  No. 
  6, 
  one 
  spot 
  of 
  growth 
  only 
  ; 
  No. 
  7, 
  

   strong 
  dark 
  green 
  growth 
  all 
  over 
  bottom 
  and 
  sides, 
  best 
  of 
  series 
  ; 
  No. 
  8, 
  

   slight 
  growth 
  ; 
  No. 
  9, 
  slight 
  growth, 
  better 
  than 
  No. 
  8 
  ; 
  No. 
  10, 
  no 
  growth 
  ; 
  

   No. 
  11, 
  fair 
  growth; 
  No. 
  12, 
  no 
  growth. 
  

  

  July 
  20, 
  1918. 
  — 
  No. 
  1, 
  no 
  growth; 
  No. 
  2, 
  fair 
  growth, 
  quite 
  obvious 
  

   difference 
  between 
  Nos. 
  1 
  and 
  2 
  ; 
  Nos. 
  3 
  and 
  4 
  (in 
  darkness), 
  no 
  growths 
  ; 
  

   No. 
  5, 
  good 
  growth 
  in 
  thick 
  discrete 
  clumps 
  ; 
  No. 
  6, 
  only 
  one 
  colony 
  com- 
  

   mencing 
  to 
  degenerate 
  ; 
  No. 
  7, 
  the 
  best 
  of 
  the 
  series. 
  Abundant 
  dark 
  green 
  

   growth 
  and 
  many 
  gas 
  bubbles 
  ; 
  No. 
  8, 
  fair 
  growth, 
  but 
  distinctly 
  less 
  than 
  in 
  

   Nos. 
  7, 
  9, 
  11 
  ; 
  Nos. 
  9 
  and 
  11, 
  both 
  show 
  three 
  or 
  four 
  times 
  as 
  much 
  green 
  

   growth 
  as 
  No. 
  8 
  ; 
  No. 
  10, 
  no 
  growth 
  ; 
  No. 
  12, 
  very 
  slight 
  growth. 
  

  

  At 
  the 
  end, 
  just 
  before 
  Kjeldahling, 
  a 
  microscopical 
  examination 
  was 
  made 
  

   of 
  Nos. 
  5, 
  7, 
  9, 
  11. 
  All 
  these 
  showed 
  almost 
  pure 
  growths 
  of 
  small 
  round 
  

   single 
  green 
  cells 
  (Chlorella) 
  ; 
  there 
  were 
  no 
  mycelia 
  or 
  spores 
  or 
  other 
  types 
  

   of 
  non-green 
  cells. 
  

  

  