2 Captain A. D. Fraser and Dr. H. L. Duke. [Dec. 19, 



were collected there and brought to Mpumu, where they were fed on a 

 healthy goat. After 1020 flies had been put on the goat it became infected 

 with Trypanosoma uniforrne. Some days afterwards Trypanosoma vivax, 

 with which wild flies had previously been shown to be naturally infected, 

 also appeared in the goat's blood. 

 The conclusions are : — 



(1) This trypanosome, which is of fairly frequent occurrence among Lake- 

 shore antelope, is Trypanosoma uniforrne. 



(2) The available evidence points to Glossina palpalis as being the carrier 

 of this species of trypanosome. 



(3) Glossina palpalis caught on the Lake-shore are naturally infected with 

 Trypanosoma uniforrne. 



The Relation of Wild Animals to Trypanosomiasis. 

 By Captain A. D. Frasek, E.A.M.C., and Dr. H. L. Duke. 



(Communicated by Sir J. B. Bradford, Sec. R.S., being an abstract of a Report 

 to the Sleeping Sickness Commission, received May 19, 1911. Received 

 December 19, 1911,— Eead February 29, 1912.) 



Wild animals were examined with the view of ascertaining whether they 

 were naturally infected with trypanosomiasis. The majority of the animals 

 were shot ; a few were captured. 



Ten waterbuck, 20 bushbuck, and 2 situtunga were obtained from within two 

 miles of the Lake-shore where the Glossina palpalis were known to be infected 

 with Trypanosoma gamhiense and vivax and were afterwards shown to be 

 infected with T. uniforrne. With the exception of four of the bushbuck 

 ordinary blood smears were made from each animal, and trypanosomes 

 morphologically indistinguishable from T. uniforrne were found in slides taken 

 from one bushbuck and one situtunga. Blood from each of the antelope was 

 injected into healthy monkeys and goats. From 3 to 5 c.c. was the usual 

 quantity of blood injected into monkeys ; 5-10 c.c. into goats. None of the 

 monkeys developed trypanosomiasis. In the blood of one of the goats 

 T. uniforrne appeared 10 days after the injection of blood of two bushbuck 

 shot near the Lake. Unfortunately no blood smears were made from these 

 animals. It is suggested that, if animals more susceptible than goats are to 

 T. uniforrne had been employed, a higher percentage of the antelope examined 

 might have been shown to harbour this species of trypanosome. 



