1911.] The Relation of Wild Animals to Trypanosomiasis. 



3 



Two bushbuck and one situtunga were also examined by feeding clean 

 laboratory-bred G. palpalis upon them and subsequently transferring the flies 

 to normal monkeys. The monkeys remained healthy and no flagellates were 

 found in the flies which were dissected. 



Twenty-two hippopotami, 8 crocodiles, 12 monitors, 60 Lake-shore birds, 

 2 bushpigs, 6 otters, etc., were examined, both microscopically and by blood 

 injection into susceptible animals, with negative results. 



G. palpalis were seen to feed on bushbuck and monitors in nature. 



The animals obtained from a distance greater than two miles from the 

 Lake-shore included 5 bushbuck, 2 duiker, and 3 bushpigs. T. ingens was 

 present in slides made from 1 bushbuck. 



Experiments were also carried out to ascertain whether bushpigs, etc., could 

 be infected with T. gambiense. Blood from monkeys heavily infected with 

 this trypanosome was injected into two young bushpigs. Cages of G. palpalis 

 known to be infected with T. gambiense were also fed upon the pigs. No 

 evidence was obtained that the pigs became infected. 



Negative results were also obtained of attempts to infect a young crocodile, 

 a monitor, a frog, and fowls. An edible rat was infected by injection. 



Conclusions. 



1. T. uniforme was the only species of trypanosome obtained as the result 

 of examination of wild animals including 32 Lake-shore antelope. 



2. The available evidence points to bushpig, crocodile, monitor, frog and 

 fowls being refractory to T. gambiense. 



3. The edible rat, which is susceptible to T. gambiense, can, by virtue of its 

 habits, be of little importance in considering the question of a reservoir. 



