148 Prof. E. Goldmann. On a New Method of [Feb. 22, 



■" pyrrhol cell," the basophile plasma cell and Ehrlich's " mast cell," distin- 

 guishable by the meta-chromatic orange colour which its granules display. 



For purposes of leucocytic and lymphocytic analysis I have found the 

 combination of the vital stain and Ehrlich's triacid mixture most useful. In 

 this instance paraffin sections of tissues taken from animals which have 

 been subjected to trypan-blue injections are necessary. 



In my experiments on tuberculosis I have succeeded in retaining the vital 

 ■stain even in sections which underwent the carbol-fuchsin staining and 

 nitric acid differentiation for tubercle bacilli. Not only did we discover 

 the bacilli, but we were able also to discriminate all the phases of dis- 

 integration, which the vitally-stained cell granules gradually underwent 

 through the intracellular multiplication of the bacilli and their effect on 

 cell structure. In this case also paraffin sections from trypan-blue animals 

 .are required. 



The number of " vital stains " recently discovered by myself and by my 

 pupil, Herr Schulemann, is rapidly increasing. Our chief object in the search 

 for new stains is to discover the exact chemical radical which produces the 

 vital reaction. We have as yet not come across a single "vital stain" which 

 differs in its general behaviour towards the living organism from that of 

 trypan- and isamin-blue. Hence, new proof has been furnished for my 

 original contention, that in intra-vitam staining we are dealing with 

 established chemical properties in the cell plasm and with an innate con- 

 stitution of its structure. Amongst the new stains which are now engaging 

 ■our attention I mention the following, appending a few remarks as to their 

 application. All stains are procurable from Grubler and Co., Leipzig. 



1. Trypan-violet. — It corresponds in all details of vital staining, applica- 

 tion, fixation, etc., to trypan-blue. The formula is : — 



OH NH 2 



