150 Prof. E. Goldmann. On a New Method of [Feb. 22, 



7. Vital " Mu Gelb."— ' 



S0 3 Na 



S0 3 TS T a 



\ 



CH 3 



8. "Dianil Blau," R.- 



HO OH 



.A. 



> 



>N = N 



Na0 3 S 



Na0 3 S 



S0 3 Na 



As to technique, I will merely add that a solution of these stains in 

 Ringer's fluid is advisable for purposes of intra-vitam staining. It is well to 

 begin experiments on animals whose toxic susceptibilities are not yet tested, 

 by injecting a weaker dose than that which results from the formula for 

 subcutaneous and intraperitoneal application — 1 cm. of a 1-per-cent. solution 

 for 20 grammes of animal's body weight. 



Evidence gathered both in physiological and pathological fields seemed to 

 point towards the fact that cell plasma, which accepts fat and lipoid stains, 

 such as sudan, scarlet red, and Nile blue, also shows a marked tendency 

 towards vital stains. But the exact relation between intracellular fat and 

 lipoid substances, on the one hand, and bodies affected by the vital stain, on 

 the other, was revealed by a careful analysis of the Pyrrhol cell (to be 

 described in full below) during its transformation into the spindle cell of 

 scar tissue and during the varied phases of its disintegration under the 

 influence of bacillary necrosis. In both cases I discovered that when 

 unimpaired, the pyrrhol cell accepts the vital stain only, whereas during 

 the progress of its transformation or disintegration its affinity for the vital 

 stain decreases and its powers of attracting the fat stains increase. Hence 

 I am inclined to assume that, under normal conditions, fat or lipoid substances 

 of the cell plasma unite with proteins and form loose compounds, liable to 

 intra-vitam staining. Once this coherence is destroyed, the histo-chemical 

 fat reaction becomes evident, whereas the vital stain is lost. 



Similar relations between fat and albumen have already been demonstrated 

 for the blood. Under normal circumstances, as long as fat is linked to 

 albumen, its extraction by ether is rendered impossible by the " protective " 



