1912.] Antelope and their Relation to Trypanosomiasis. 



169 



T. vivax in Goat 512. 



maxim., 27"0 //,; minim., 20 /jl ; average, 23'9 /jl (osmic acid fixation). 

 T. gambiense in Goat 512. 



T. vivax and T. gambiense in centrifugalised blood of Goat 512. 

 (3) Sub-inoculation. 



The blood of Goat 512 inoculated into Monkey 525 produced T. gambiense 

 in the blood of the monkey. 



Conclusions. 



(1) That the continued infectivity of the wild G. palpalis on Damba Island 

 to monkeys may be explained by the fact that the situtunga on that island 

 are acting as a reservoir to T. gambiense. 



(2) That the continued infectivity of the mainland flies to T. gambiense 

 may probably be explained on a similar hypothesis, including possibly the 

 other species of antelope frequenting the lake shore, i.e. waterbuck, bushbuck, 

 duiker and reedbuck. 



(3) That no positive evidence can be adduced from the above experiments 

 to show that hippopotamus can serve as a reservoir. 



(4) That the continued infectivity of the lake-shore flies to T. vivax and 

 T. uniforme is also due to the antelope which serve as a reservoir for these 

 trypanosomes. 



DESCRIPTION OF PLATE. 

 (All drawn at magnification of 2000 diameters.) 



a. T. uniforme from blood of Situtunga 511. Fixed by drying only followed by absolute 



alcohol. 



b. T. vivax from blood of Goat 512. Fixed with osmic acid followed by absolute alcohol. 



d. T. gambiense from blood of Monkey 401, which was infected from Situtunga 402-403. 



Fixed osmic acid followed by absolute alcohol. 



e. T. gambiense from blood of Monkey 504, infected by laboratory-bred glossinee from 



Monkey 401. Fixed osmic acid followed by absolute alcohol. 

 /. T. gambiense from blood of Eat 477, infected from Monkey 401. Fixed osmic acid and 

 absolute alcohol. 



cf. T. gambiense from blood of Monkey 511, infected from Situtunga 509 -510. Fixed 

 osmic acid and absolute alcohol. 



VOL. LXXXV. — B. 



N 



