1912.] Reservoir for Trypanosoma gambiense. 301 



Fly Experiments. 







Number of flies. 



Length 



Eesult 



of 

 feeding 



clean 

 monkey. 





Expt. 

 No. 



Period for which 

 flies fed upon 2357. 



1st 

 day. 



30th 

 day. 



Dissected 

 during 

 experi- 



Con- 

 taining 



of 

 experi- 

 ment in 



Remarks. 



53 

 207 



282 

 532 



May 12— 20 & „ 

 July 18 — 25 „ 



Aug. 10—16 „ 

 Nov. 25—30 „ 



29 

 34 

 98 



91 

 64 



25 

 31 

 87 



75 

 39 



29 

 33 

 98 



91 

 64 





 

 1 





 



52 

 48 



53 

 38 



- 



41st day fly. Only gut 

 infected. Tryps. + + + 



Never fed on clean mon- 



535 



Nov. 29— Dec. 5 



89 



61 



89 











Fed upon monkey through- 

 out experiment. 





Totals 



405 



318 



404 



1 























The flagellated fly obtained in Experiment 207 was remarkable in showing 

 negative proventriculus and salivary glands, although dissected on the 

 41st day of the experiment. 



Injection Experiments. 



Date. 



Expt. No. 



Quantity of blood injected. 



Eesult. 



Animal used for 



Pure. 



Citrated. 



injection. 



14.12.11 



24.1.12 



569 

 630 

 649 



8 

 10 



cc 

 6' 





Monkey. 



The citrated blood mentioned in the above, and also in the ensuing 

 injection experiment, consists of an admixture of the freshly drawn blood 

 with a citrate solution consisting of sodium citrate 1 per cent, and sodium 

 chloride 0"8 per cent. The mixture is arranged so that the blood just loses 

 its power of clotting, the proportion being about three parts of blood to two 

 of citrate. This antelope was thus shown to harbour T. garribie r iise 15 months 

 after its original infection. Since that date, i.e., a period of seven months, no 

 positive evidence has been obtained. 



Eeedbuck 2431. 



Original infection by laboratory-bred flies carrying human strain of 

 T. gambiense, May, 1910. Latest evidence, previous to this discussion, of 



