302 Dr. H. L. Duke. Antelope as a [Apr. 20, 



the infectivity of this antelope obtained in November, 1910, when it proved 

 infective to laboratory-bred G. palpalis. 



Fly Experiments. 



Expt. 

 No. 



Period for which flies 

 fed upon 2431. 



Number of flies. 



Length 



of 

 experi- 



day, 



Eesult 



of 

 feeding 



Remarks. 



1st 

 day. 



30th 



Dissected 

 during 



ment. 



Con- 



flagel-° 

 lates. 



93 

 217 



314 

 586 



590 



May 29— June 5, 1911 

 July 22— 29, 1911 ... 



Aug. 21— 26, „ 

 Dec. 26—31, „ 



Dec. 28, 1911— Jan. 1, 

 1912 



54 

 93 



76 

 73 



73 



89 



55 

 42 



51 



54 

 93 



73 

 73 





 





 







51 

 46 



51 

 31 



34 





Fed on monkey through- 

 out the experiment. 



Never fed upon clean 

 monkey. 





Totals 



369 



280 368 













Experiments 93 and 314, 586 and 590, were all nourished on fowl's blood 

 during the first 30 or so days of the experiment. In Experiment 93 one fly 

 was found infected with flagellates ; these, however, as will be pointed out on 

 another occasion, were derived from the fowl, which was found infected with 

 T. gallinariim. 



Injection Experiments. 



Date. 



Expt. No. 



Quantity of blood injected. 



Eesult. 



Animal used for 



Pure. Citrated. 



1 



injection. 



23.1.12 



16.2.12 



629 

 656 



6* 

 6 





Monkey. 











Since November, 1910, therefore, during a period of 15 months, Reed- 

 buck 2431 has given no evidence of infectivity to T. gamliense. Attempts 

 will be made forthwith to reinfect this antelope first with flies and sub- 

 sequently by direct blood injection. In the event of a negative result the 

 serum reactions will then be investigated. 



