386 



Messrs. Ellis and Gardner. Origin and 



[May 9, 



very small quantities of extracted bran. It retained a healthy condition and 

 constant weight. 



The blood, of which 10r3 grm. were obtained, yielded as total cholesterol 

 0'2440 grm. of digitonin cholesteride, and as free cholesterol - 2302 grm. of 

 the compound. These figures represent a percentage of - 0552 free cholesterol 

 and 0-0033 ester cholesterol. 



The kidneys were cut in a few pieces, rinsed with saline, and freed from 

 adhering moisture by means of blotting paper. They weighed 2072 grm., 

 and yielded as total cholesterol 01390 grm. of digitonin cholesteride, and as 

 free cholesterol 01080 grm. of the compound. These figures represent a 

 percentage of 0*2534 free cholesterol and 0-0728 ester cholesterol. 



Muscle. 100 grin, were taken for the estimation, the sample being cut 

 away from the body wall and limb muscles. The total digitonin cholesteride 

 was - 1545 grm. and the free was 0-1360 grm., corresponding to a percentage 

 of 0-0661 free and 0-0090 ester cholesterol. 



The lungs were also washed with saline and freed from adhering moisture 

 by means of blotting paper. Their weight was 911 grm. The total 

 digitonin cholesteride was 0-0765 and the free was 0*0473 grm., correspond- 

 ing to a percentage of 0-2524 free and 01558 ester cholesterol. 



The brain, dissected out as completely as possible, weighed 10 - 592 grm. "It 

 was dried with sand and plaster of Paris in the usual manner. One-quarter 

 Of the ether extract was taken for the free cholesterol estimation, and half of 

 the extract was saponified, of which one-half was taken. This was found 

 advisable as it was more convenient to deal with small quantities of the com- 

 pound. The total digitonin cholesteride was 0*2110 grm. and the free - 2310. 

 The free cholesterol was thus 212 per cent., and no ester was present. 



Rabbit B received the same diet as A of the previous experiment. It was 

 fed for a period of about seven weeks, and appeared in a normal healthy 

 condition when killed. As the organs were treated in a precisely similar 

 manner to those of the previous experiment, we shall merely give the figures 

 obtained from the analysis. 



The blood; 89 - 6 grm. were taken for the estimation. Half the ether 

 extract yielded 0-1285 grm. of cholesteride, which corresponds to 0-0697 per 

 cent, free cholesterol, and the saponified half yielded 01429 grm. of 

 cholesterol, corresponding to a total of - 0775 per cent, cholesterol and, by 

 difference, 0*0078 ester cholesterol. 



The kidneys weighed 15"65 grm. Half the extract yielded 0-0505 grm. of 

 cholesteride, equal to 01568 per cent, free cholesterol. The saponified half 

 yielded 0*0857 grm. of cholesteride, equal to 0-2661 per cent, total cholesterol 

 and, by difference, 0'0093 per cent, ester cholesterol. 



