388 



Messrs. Ellis and Gardner. Origin and 



[May 9, 



The kidneys weighed 14-645 grm. The unsaponified half yielded 

 - 0629 grm. of cholesteride, equal to 02296 per cent, free cholesterol. The 

 saponified half yielded 0-0842 grm. of the cholesteride, equal to 0*2794 per 

 cent, total cholesterol, and, by difference, 0-0498 per cent, ester cholesterol. 



The muscle; 100 grm. were taken. The unsaponified half yielded 

 0'0928 grm. of cholesteride, equal to - 0451 grm. of free cholesterol. The 

 saponified half yielded 0-1075 grm. of the cholesteride, equal to 0-0522 per 

 cent, of total cholesterol, and, by difference, 0-0071 per cent, of ester 

 cholesterol. 



The lungs weighed 12-25 grm. Half of the unsaponified half yielded 

 0-0446 grm. of cholesteride, equal to - 3538 per cent, free cholesterol. Half 

 of the saponified half yielded 0-0622 grm. of cholesteride, equal to - 2488 per 

 cent, total cholesterol. The ester cholesterol was, by difference, therefore, 

 0-0404 per cent. 



The brain weighed 9 - 8 grm. One-half of the unsaponified half yielded 

 0-2892 grm., equal to 2 - 3368 per cent, free cholesterol. There was no ester 

 cholesterol. 



Experiments VII and VIII: — 



In this case the cholesterol was injected in olive oil solution into the 

 peritoneal cavity. 1 grm. was injected. A control experiment was perform ed 

 injecting pure olive oil only. Both animals lost about 1 kgrm. in weight. 



The blood; the control yielded 76 - 55 grm. Half the extract yielded 

 - 0963 grm. cholesteride, equal to 0611 per cent, free cholesterol. The 

 saponified half yielded 0-0963 grm. cholesteride, equal to 0637 per cent, 

 total. By difference the ester cholesterol is - 0026 per cent. The cholesterol- 

 injected animal yielded 81 - 895 grm. of blood. Half the saponified extract 

 gave 0-1435 grm. cholesteride, equal to - 0849 per cent, total cholesterol. 

 The ester was not estimated through an accident. 



The kidneys; the control kidneys weighed 14-53 grm. The unsaponified 

 extract yielded 0-0565 grm. cholesteride, equal to 0*1890 per cent, free 

 cholesterol. The saponified half yielded 0-0892 grm. cholesteride, equal to a 

 total of 0-2985 per cent, cholesterol. The ester cholesterol was, therefore, 

 0-1095 per cent. 



The kidneys from the cholesterol-injected animal weighed 15*98 grm. The 

 unsaponified whole extract yielded 0*1050 grm. of cholesteride, equal to 

 0-1597 per cent, free cholesterol, and the filtrate after saponification 0*1227 

 per cent, cholesteride, equal to 0*1966 per cent, ester cholesterol. The total 

 is, therefore, 0*3562 per cent. 



Muscle; 150 grm. were taken from the control rabbit. The unsaponified 



