390 Messrs. Ellis and Gardner. Origin and [May 9, 



0-4562 per cent, total cholesterol. The ester cholesterol was therefore, by- 

 difference, 0-0698 per cent. 



The muscles; from I were taken 125-5 grm. The unsaponified half of the 

 extract yielded 0*1585 grm. of cholesteride, equal to 0-0614 per cent, free 

 cholesterol. The saponified half yielded 0-1793 grm. of cholesteride, equal to 

 0-0694 per cent, total. The ester cholesterol was, by difference, therefore, 

 0-0080 per cent. 



From J HO'58 grm. were taken. The unsaponified half yielded 

 0-1680 grm. of cholesteride, equal to 0"0738 per cent, free cholesterol. 

 The saponified half yielded 0-1690 grm., equal to 0-0743 per cent, total 

 cholesterol. The ester cholesterol was, therefore, 0-0005 per cent. 



The lungs from Babbit I weighed 8-97 grm. The unsaponified half of the 

 extract yielded 0-1536 grm. of the cholesteride, equal to 0-4246 per cent, 

 free cholesterol. The saponified half yielded - 1637 grm., equal to - 4526 per 

 cent, total cholesterol. The ester was, therefore, - 0279 per cent. 



From Eabbit J the lungs weighed 5 - 155 grm. The saponified half yielded 

 0-0669 grm. of cholesteride, equal to - 6307 per cent, free cholesterol. There 

 was a trace of ester cholesterol. 



The brain from Eabbit I weighed 1089 grm. One-fourth of the extract 

 yielded 0-2682 grm. of the compound, corresponding to 2-3938 per cent, free 

 cholesterol. There was no ester. 



From J the brain weighed 10 - 59 grm. One-fourth of the extract yielded 

 0-2310 grm. of the compound, corresponding to 2-0617 per cent, free 

 cholesterol. There was no ester cholesterol. 



Bile. — Attempts were made to estimate the cholesterol content of the bile 

 of the rabbits under different diets and during starvation. In the case of 

 the normal animals, whether fed with green food or extracted bran, it was 

 possible to detect cholesterol, but quite impossible to estimate gravimetrically 

 with any approach to accuracy. The amount of bile in the gall bladders in 

 each case was only - 5 — 1 grm. in weight. In one or two cases the results 

 were interesting. The contents of the gall bladder of the cholesterol and 

 olive oil injected rabbit was found to be quite solid. Its weight was 

 T72 grm. and contained T266 per cent, free cholesterol and no ester. The 

 bile of the rabbit injected with olive oil only weighed about 3 - 66 grm. and 

 contained - 081 per cent, total cholesterol with a trace of ester cholesterol. 

 In the former case the large excess of cholesterol in the bile was not wholly 

 re-absorbed; an examination of the faeces from the animal showed that 

 7'3 mgrm. of cholesterol was excreted per day, as mentioned in our previous 

 communication. 



The biles from the two rabbits kept in a state of inanition contained 



