410 Dr. E. W. A. Walker. [May 16, 



Table VIII. — Showing the Eeactions of some of the Streptococci from Table V 

 after Four Months' Cultivation in Mannite Medium. 



Three are identical, and V differs only in regard to the clotting of milk. 



Streptococcus. 



Milk clot. 



1 

 1 



1 



Eaffinose. 



Inulin. 





Mannite. 



L 











+ 















P 











+ 







°o 



+ 







M 











+ 











+ 







V 









+ 











+ 







s 



























The 1912 reactions of the streptococci L, P, G, M, V (all of human derivation) 

 tested after 18 months' continuous cultivation in stab agar further support 

 the hypothesis, since with the exception of two differences in the saccharose 

 reaction they are seen in Table IX to present an absolute identity. 



'Table IX. — Showing the Eeactions of some of the Streptococci from Table V 

 after 18 Months' further Cultivation in Stab Agar. 

 TJiree are identical, and the other two differ only in regard to the saccharose 

 reaction. The reason why the reactions differ from those obtained with agar 

 •cultures in 1910 is unknown. It is not known, for example, whether the agar 

 has undergone any change. The leading reactions are the same as those of P 

 from agar in 1908. 



L + + + + 



P + + + + 



M + + + + 



G- + + + + + 



V + + + + + 



Accordingly I conclude that the tests may assist in the determination of the 

 source and origin of particular strains of streptococci met with. It is, 

 however, necessary to speak with a good deal of reserve upon this point 

 because at present we do not possess enough exact evidence as to the length 



