420 Mr. V. J. Harding. [May 21, 



The following mixtures were incubated for 48 hours at 37° in presence of 

 toluene, and the free phosphate estimated as before : — 



A. 5 c.c. autolysed ox pancreas + 15 c.c. water. 



B. „ „ +15 c.c. hexosephosphate. 



C. The same as B, but the pancreas mixture employed was boiled. 

 Original hexosephosphate = 0"5351 grm. Mg 2 P 2 7 . 



Free phosphate in A = 0*0 „ 



B = 0-0915 



C = 0-0814 



Phosphate liberated = 0-0101 



Practically no action had taken place. 



(c) Emulsin. — The emulsin was prepared as described by H. E. and E. F. 

 Armstrong and Horton.* 



The experiments were carried out at 37° in presence of toluene, the 

 following mixtures being employed : — 



A. 10 c.c. emulsin solution + 15 c.c. water. 



B. „ „ +15 c.c. hexosephosphate. 



C. Same as B, but boiled emulsin employed. 

 Original hexosephosphate = 0-6274 grm. Mg 2 P 2 7 . 





After 



48 hours. 



144 hours. 



„ B 



C 



Phosphate set free by enzyme ... 



o-o 



-1750 

 -0342 

 -1408 



o-o 



-3064 

 -0563 

 -2501 



Emulsin has, therefore, a considerable hydrolytic action on hexose- 

 phosphate. 



(d) Zymin Extract. — 10 grm. of " zymin " preparation were digested four 

 days with 30 c.c. water at 25°. The resulting mixture was filtered and used 

 in the experiment. It was found to contain considerable amounts of 



An aqueous extract of zymin (yeast treated with acetone, was found only 

 to have a slight action on hexosephosphate. This is seen from the following 

 experiment carried out at 25° in presence of toluene, the mixture ' being 

 incubated for three days : — 



* 'Eoy. Soc. Proe.,' 1908, B, vol. 80, p. 324. 



