422 The Action of Enzymes on Hexosephosphate. 



The mixtures A, B, C were incubated with toluene at 25° for 24 hours : — 



A. 10 c.c. above solution + 15 c.c. water. 



B. „ „ +15 c.c. hexosephosphate. 



C. As B, but enzyme solution previously boiled. 



Original hexosephosphate = 0-5665 grm. Mg 2 P20 7 . 



After incubation, free phosphate in — 



A = 0-0494 

 B = 0-1268 

 C = 0-0755 



Phosphate set free by enzyme =0-0513 „ 



The hexosephosphatase, therefore, can be precipitated by means of alcohol 

 and ether. 



Summary. 



(a) Ricinus lipase and emulsin possess a slow hydrolytic action on 

 hexosephosphate. 



(6) Autolysed ox pancreas is almost without action upon hexosephosphate. 



(c) " Zymin " extract hydrolyses hexosephosphate slowly. 



(d) Autolysed yeast-juice possesses a marked action on hexosephosphate. 



(e) An alcohol-ether precipitation of autolysed yeast-juice possesses 

 considerable activity towards hexosephosphate. 



