22 Mr. C. H. Kellaway. The Effect of Certain 



Table IX. — The Cholesterol-content of the Blood and Adrenals of Normal 

 and Polyneuritic Pigeons. 





Diet. 



Number 



of 

 days on 

 diet. 



Initial 

 body 



weight 

 in 

 grm. 



Weight 



of 

 adrenals 

 in mgrm. 



Cholesterol- 

 content of 

 adrenals 

 in mgrm. 



Percentage 

 of 



cholesterol 

 in 



adrenals. 



Weight of 

 cholesterol 



on 1 c.c. 



of blood 

 in 



mgrm. 



Average 



Normal 





314 -6 



25-5 



0-70 



2-9 



1 66 



Maximal 









32 -0 



0-88 



3-8 



2 -46 



Minimal 









21 



0-44 



2-0 



1 -06 



Average 



Polished 



18 



320 



47 -7 



0-79 



1 -9 



3-06 



Maximal 



rice 







66 



1 -34 



3-3 



3-69 



Minimal 









32 -0 



0-35 



0-67 



2-40 



evidence of storage in the suprarenals. These results do not lend any 

 support to the hypothesis of lipoid storage as far as cholesterol is concerned. 

 Some observations were also made on rabbits and pigeons fed on a normal 

 diet with the addition of cholesterol, in order to ascertain if the hyper- 

 cholesteraemia produced in this way was associated with increase in the 

 content of adrenaline in the glands. 



Feeding Experiments with Cholesterol. 



Eleven healthy rabbits, two months old, were selected for experiment. Of 

 these, six were fed daily through a catheter with 0"1 grm. of pure cholesterol 

 dissolved in 2-5 c. c, of olive oil. All the rabbits had the same basal dietary, 

 consisting of bran, oats, and occasional greens. The experiment was continued 

 for nearly seven weeks. All the rabbits remained healthy and gained weight, 

 except one of the experimental animals, which became paraplegic after forty- 

 two days' feeding, and which was discarded. At autopsy the animals fed with 

 excess of cholesterol showed aortic lesions like those described by Anitschow 

 and Chalatow, but only two had any adrenal hypertrophy. 



Before the rabbits were killed, a sample of blood was taken from the ear 

 for cholesterol estimation. The adrenals were carefully dissected out post 

 mortem, weighed, and the residual adrenaline was estimated colorimetrically. 



The average results obtained from the two series of rabbits are given in 

 Table X. 



There is a small increase in the content of adrenaline of the rabbits in 

 which hypercholestersemia had been produced and maintained, above the 

 average content of normal rabbits investigated at the same time. A more 

 rigid control would have been provided had these latter animals been fed 

 daily with 2*5 c.c. of olive oil without cholesterol. 



