Dietary Deficiencies on the Suprarenal Glands. 9 



Table I. — Comparison of Methods of Estimating Adrenaline. 





Weight of 



"Weight of 



Weight of 





Initial 



adrenals 



adrenaline in . 



adrenaline in 





weiglit in 



in 



milligrammes 



milligrammes 



Eemarks. 



grammes. 



milli- 



determined 



determined 





grammes. 



physiologically. 



colorimetrieally. 





350 



21 "0 



'028 



"030 



Normal. 



4.50 



19 "0 



"028 



-031 





320 



31 '0 



-095 



-095 



, „ (some time in the 











laboratory). 



319 



16 -0 



0-045 



0-046 





319 



27 -0 



-060 



-056 





440 



67-0 



0-105 



-103 



Pigeon with polyneuritis fed on 











polished rice. 



I 430 



71 -0 



0-086 



0-090 



)» »i 



420 



55-0 



0-168 



-159 



)» 



1 345 



20 -0 



0-105 



0-115 1 



Pigeons without symptoms fed 



1 

 1 









on polished rice + 1 grm. 



1 340 



25 -0 



0-133 



0-128 J 



caseine + 1 grm. marmite daily. 



i 





(fig- 1)- 





Fig. 1. — Physiological estimation of adrenaline in the suprarenal glands of a pigeon fed 

 on polished rice + 1 grm. marmite and 1 grm. of casein daily. C and E = response 

 to 0-35 c.c. of extract (total volume = S'l c.c). A, D, and F = response to 

 0-0098 uigrm. B, to 0-U084 mgrm. of adrenaline. Total content of glands 

 = 0-133 mgrm. of base. 



For quantities of the order estimated the differences observed are within 

 the limits of exj)erimental error. 



In a certain number of birds the adrenals were estimated separately, and 

 in normal birds, even when there was some difference in weight between the 



