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Dictyohinesis in Germ Cells, or the Distribution of the Golgi 

 Apparatus during Cell Division* 



By E. J. LuDFOKB, B.Sc. (Lond.), and J. Bronte Gatenby, B.A., B.Sc, 



D.Phil. (Oxon.). 



(Communicated by Prof. J. P. Hill, F.R.S. Received April 20, 1921.) 

 [Plates 17 and 18.] 



Introduction. 



In the vast majority of animal cells so far properly studied, two categories 

 of cytoplasmic inclusions have been identified, namely, the mitochondria and 

 the Golgi apparatus. 



The Golgi apparatus generally takes the form of an excentric juxta- 

 nuclear system or network, composed of rodlets, platelets or beads, arranged, 

 in many cases, around and over the surface of the centrosphere or archoplasm, 

 in which lies embedded the centrosome. In highly differentiated cells such 

 as the oocyte or nerve ganglion cell, the Golgi apparatus becomes dispersed 

 into the farthermost parts of the cell-cytoplasm, and in most cases therefore 

 loses its relationship to the centrosome. 



The equipment of an ordinary cell so far as cell organelles are concerned, 

 may be stated to be : — (a) chromosomes, (5) nucleoli, (c) Golgi apparatus, 

 {d) mitochondria, {e) centrosome and centrosphere. During cell division or 

 cytokinesis, all these bodies or organellse are distributed between the 

 daughter cells in some special manner. In this paper we have investigated 

 carefully for the first time the fate during mitosis of the internal apparatus 

 of Golgi, in a number of animals, from both Vertebrate and Invertebrate 

 Phyla. 



Material and Methods. 



The material used had been prepared by one of three methods : a formalin- 

 silver nitrate, Golgi apparatus technique; a Kopsch method, or chrome 

 osmium and iron alum hfematoxylin. The Cavia material was prepared by 

 the Mann-Kopsch-Altmann technique (Gatenby), the Mus testis by Da Fano's 

 cobalt nitrate formalin and silver nitrate modification of Cajal's method, the 

 Stenobothrus testis by the latter technique (Cajal), and the Mollusc gonads 

 by Kopsch, and by the chrome osmium methods. It will be clear therefore 



* Pait of the materials used for this research were purchased by a Government Grant 

 of the Royal Society, for which we express thanks. 



VOL. xcn. — B. T 



