UcBinolytic Activity of Chemical Substances. 291 



1. Saponin. 



The following values were obtained for the constants in the equations 

 relating to T and t : — 



6. 



a. 



/3. 



y- 



5000 



9-4 



25 



4-1 



10000 



10-4 



57 



8-3 



20000 



12-4 



139 



16-6 



30000 



14-4 



252 



25 



From these, the following values are calculated for the constants in the 

 equation relating «, /3 and 7 to 8 : — 



a. b. ti. m. n. p. 



1300 ' 05 0-4 0-0002 8-4 0-00083 



The correspondence between observed and calculated results is shown by 

 the following Tables : — 



8 = 5,000. 8 = 20,000. 



r. 



Texper. 



Tcalc. 



T. 



Texper. 



Tcalc. 



10 



2-3 



2-5 



12 



16 



15 



30 



0-17 



0-2 



40 



0-4 



0-5 



g = 50,000. 



T. Texper. Tcalc. 



40 2-3 2 



2. Soduim Taurocholate. 

 Certain points have to be noted regarding this salt. 



(1) A pure dry specimen must be obtained. Dryness is ensured by 

 desiccating in vacuo for about a fortnight. 



(2) Sodium taurocholate does not form a true solution. In saline, water, 

 or dilute alcohol, it becomes opalescent, and when in this state will all 

 pass through a fine filter. Its behaviour is similar to that of a soap. 

 The rapidity with which this opalescence forms depends on the dilution, a 

 1 per cent, solution remaining clear for some time, while a O'l per cent, 

 solution becomes rapidly cloudy. The stability is lessened by heating. 

 Consequently it is necessary (a) to make the original solution, from which 

 dilutions are to be made, not more dilute than 1 per cent. ; (b) to make the 

 dilutions and perform the experiments as rapidly as possible ; and (c) to avoid 

 heating the solution unnecessarily. 



VOL. XCIL— B. Z 



