1908.] Destiny of Cholesterol in the Animal Organism. 123 



experiments, but we think the actual determinations are of sufficient interest 

 and accuracy to be placed on record. The method we adopted for the brain 

 and cord was to mix with plaster of Paris in a mortar, and, after the mass 

 has hardened, to powder it thoroughly. This was then thoroughly extracted 

 with ether (21 days) and the extract treated in the usual way. The rest of 

 the rabbit carcase (including the fur) was finely minced in a machine, mixed 

 with plaster of Paris, and again passed through the machine. After it had 

 set to a dry mass it was ground up in a mortar with coarse sand and plaster 

 of Paris, and extracted for three weeks with ether. The results are collected 



together in the following table 



Table II. 





Weight 

 in grammes. 



Unsaponiiiable 

 matter. 



Pure 

 cholesterol 

 found. 



Cholesterol 

 per cent. 



Babbit A, weight 2 - 5 kilos., fed on extracted bran for 20 days. 



Blood 



73 



0-14 



(trace) 





Brain and spinal cord 



14 3 



0-68 



0-427 



3 



Best of rabbit 



2413 



3-75 



2-168 



0-09 



Rabbit B, weight 2 -2 kilos., fed on extracted bran +2\ grammes of cholesterol 





for 10 days. 







Blood 



71 



0-29 



-0295 



-0415 



Brain and spinal cord 



13 -31 



1 -31 



(lost) 





Best of rabbit 



2116 



3 -50 



1 -908 



0-09 



Rabbit C, weight 2 - 8 kilos., fed on a mixed diet of cabbage, oats, and bran 



for 1 month. 



Blood 75 0-117 I (trace) I — 



Brain and spinal cord 17 5 t -776 -5225 3 "0 



Best of rabbit , 2708 5-11 2-716 -10 



Six rabbits, fed on above mixed diet. 



Blood I 500 0-464 j 0-1199 i 0-024 



Experiments to ascertain whether the Cholesterol Content of the 

 Blood can be Correlated with Variation in the Cholesterol 

 Content of the Food in Carnivorous Animals. 



A. Experiments in which the Animals toere killed two to four hours after 



a Meal. 



Experiment XII. — A dog, weighing 7 '3 6 kilogrammes, was fed for 10 days 

 on a daily ration of 200 to 300 grammes of bread, the whites of two eggs, 



