124 Messrs. Doree and Gardner. Origin and [Dec. 18, 



and a teaspoonful of cream, the whole being moistened with a solution of 

 Liebig's extract of beef, and lightly fried. The animal was killed three and 

 a half hours after the last meal. The blood weighed 480 grammes, and from 

 this 0*687 gramme of unsaponifiable matter was obtained. This was at 

 once benzoylated in pyridine solution, and a total crop of cholesterol benzoate 

 weighing 0*4865 gramme was separated. This corresponds to 0*3892 gramme, 

 or 0*0811 per cent, of cholesterol. 



A post-mortem examination showed that the stomach was practically empty. 

 The gall-bladder contained 5*2525 grammes of bile which on evaporation 

 yielded 0*892 gramme of solid matter. From this 0*002 gramme of 

 cholesterol benzoate was obtained, or 0*18 per cent, of cholesterol (calculated 

 on the dry solids). 



Experiment XIII. — A dog, weighing 8*13 kilogrammes, was fed for nine 

 days on a daily ration of 250 grammes of raw brain. At first it did not 

 take kindly to this food, but in the last few days it consumed the whole of 

 the brain given. The animal was killed two hours after a meal. The 

 weight of blood obtained was 430 grammes, which yielded 0*74 gramme of 

 unsaponifiable residue. This was benzoylated directly in pyridine solution, 

 and 0*484 gramme of pure cholesterol benzoate was obtained, melting 

 correctly. This corresponds to 0*3872 gramme, or 0*09 per cent, of 

 cholesterol. 



A post-mortem examination showed that the stomach contained some 

 undigested food. The gall-bladder contained 2-06 grammes of bile of a pale 

 yellow colour, and left 0*2245 gramme of solid matter. From this 

 0*004 gramme of unsaponifiable matter was obtained, but only a trace of 

 benzoate could be separated from it. 



Experiment XIV. — A dog, weighing 9*77 kilogrammes, was fed for nine 

 days on a daily ration of 200 grammes of dry oatmeal made into porridge 

 with water. The animal ate about half the last meal only, and was killed 

 four hours afterwards. The blood weighed 680 grammes and yielded 

 0*91 gramme of unsaponifiable matter. This, on benzoylation in pyridine 

 solution, gave 0*6775 gramme of pure cholesterol benzoate, corresponding to 

 0*542 gramme, or 0*0797 per cent, of cholesterol in the blood. 



A post-mortem examination showed that the stomach contained a quantity 

 of undigested food. The gall-bladder contained 6*36 grammes of bile which, 

 on evaporation, yielded 1*1065 grammes of solid residue. From this only 

 0*001 gramme of cholesterol benzoate could be obtained, corresponding to 

 0*07 per cent, of cholesterol. 



Experiment XV. — A dog weighing 8*5 kilogrammes was fed for six days on 

 a daily ration of 200 to 300 grammes of brain, mixed with some bread. The 



