1908.] Destiny of Cholesterol in the Animal Organism. 125 



animal was killed four hours after the last meal. The blood was unfortunately 

 lost. The gall-bladder contained 702 grammes of bile which, on evaporation, 

 yielded l - 3947 grammes of solid residue. From this, 0'0028 gramme of 

 cholesterol benzoate was obtained, or - 16 per cent, of cholesterol. 



B. Experiments in which the Animals were killed 24 hours after a Meal. 



Experiment XVI. — A dog weighing 7*8 kilogrammes was fed for 7 days on 

 porridge made by boiling about 100 grammes of oatmeal with water, daily. It 

 was killed 24 hours after the last meal. The quantity of blood obtained was 

 524 grammes, from which - 9845 gramme of greasy unsaponifiable matter 

 was obtained. The first crop of crystals from alcohol weighed 0'2915 gramme, 

 and a microscopic examination showed that these consisted of practically 

 pure cholesterol. The residues were benzoylated in pyridine solution and 

 yielded 0"l795 gramme of crystals, which melted correctly and gave the 

 characteristic colour play of cholesterol benzoate. The total cholesterol 

 obtained was therefore 0*435 gramme or - 083 per cent. 



A post-mortem examination showed that the stomach was quite empty. 

 The gall-bladder was distended and contained 4 - 55 grammes of bile, which, 

 after drying at 100° C, left 0"8257 gramme of solid matter. We attempted 

 to estimate the cholesterol in this quantitatively, but the amount was too 

 small for an accurate determination. However, 0'0025 gramme of choles- 

 terol benzoate was isolated, or 0'24 per cent, of the total solids. 



Experiment XVII. — A dog weighing 9 - l kilogrammes was fed for 14 days on 

 raw brain. It was given 300 to 500 grammes of fresh brain per day, but the 

 animal did not take the food very well and often left a portion uneaten. On 

 the last day of the period the dog developed feverish symptoms and was 

 killed 24 hours after the last meal. The quantity of blood obtained was 

 507 grammes and yielded T43 grammes of unsaponifiable matter. This 

 was not completely soluble in alcohol. The first crop of crystals weighed 

 - 5075 gramme and melted at 145° C. A second crop weighed 0'0785 gramme 

 and melted at 144° C. From the residues on benzoylation 0'2115 gramme of 

 cholesterol benzoate, melting at 145°, was isolated. The total cholesterol 

 obtained was therefore - 7526 gramme, or - 1486 per cent. 



A post-mortem examination showed that the stomach was empty and 

 distended with gas, and the lungs appeared to be congested. The gall-bladder 

 contained - 844 gramme of bile, which was very thick and stringy, and 

 contained - 1617 gramme of solid matter. From this - 0025 gramme of 

 cholesterol benzoate was obtained, or 1*2 per cent, of the total solids. 



Experiment XVIII. — A large dog weighing 19'2 kilogrammes was fed for 

 24 days on raw brain together with a little bread. This animal took the 



