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Messrs. Doree and Gardner. Origin and [Dec. 18, 



food readily and consumed daily from 500 to 800 grammes of brain, and at 

 the end of the period was in good health. It was killed 24 hours after 

 a full meal. The weight of blood obtained was 1140 grammes, which yielded 

 1*5 grammes of unsaponifiable matter. On crystallisation from alcohol a first 

 crop, weighing - 304 gramme, melting at 144° to 145°, was obtained, and 

 a second weighing 0*184 gramme and melting at the same temperature. The 

 mother liquors and residues, on benzoylation, gave 0*41 gramme of choles- 

 terol benzoate. The total cholesterol obtained was therefore - 816 gramme, 

 or - 072 per cent. 



A post-mortem examination showed that the stomach was empty. The 

 gall-bladder contained 15*82 grammes of bile, which gave 3'947 grammes of 

 solid matter, and from this - 017 gramme of cholesterol benzoate was 

 obtained, or - 34 per cent, of cholesterol. 



Experiment XIX. — A dog weighing 13 - 4 kilogrammes was fed for 10 days 

 on a diet practically free from cholesterol. The daily ration consisted of 

 250 to 300 grammes of bread, the whites of two eggs, two teaspoonfuls of 

 cream lightly fried together after moistening with a solution of Liebig's 

 extract of beef. The animal continued in good health and was killed 

 24 hours after the last meal. The blood weighed 760 grammes and yielded 

 T326 grammes of unsaponifiable residue. On crystallising from alcohol the 

 first crop weighed 0'4615 gramme and melted at 145°, the second weighed 

 0"2135 gramme and the same melting point. After benzoylation, the 

 residues yielded 0"1728 gramme of cholesterol benzoate, melting at 145°, 

 and showing the colour changes. The total cholesterol obtained was there- 

 fore 0*8132 gramme, or - 107 per cent. A post-mortem examination showed 

 that the stomach was empty. The gall-bladder contained 10 - 29 grammes of 

 bile which, on evaporation, gave 2 - 632 grammes of solid matter. From this, 

 0"003 gramme of cholesterol benzoate was obtained, or - 09 per cent, of 

 cholesterol. 



These results are collected together in Table III. 



The experiments differ fundamentally from those carried out on the 

 rabbit described in the earlier part of this paper. In the case of these 

 animals a standard diet free from cholesterol and similar bodies, to which 

 measured portions of cholesterol could be added, was available ; and as they 

 are practically continuous feeders the bile flow and consequently the 

 cholesterol content of the blood, due to this source, would remain practically 

 constant. In the case of the dogs we had no such cholesterol-free standard 

 diet and were obliged to make use of a number of entirely different foods, , 

 differing as far as possible in cholesterol content, though even the non- 

 cholesterol ones contained phytosterol. Little is known concerning the 



