1909.] Destiny of Cholesterol in the Animal Organism. 233 



volume of a 5-per-cent. suspension of rabbit's blood corpuscles. The results 

 of the experiment are embodied in the following table : — 



Table I. 



Amount of blood, 

 5-per-cent. suspension. 



Amount of saponin, 

 "00 L per cent. 



Amount of NaCl, 

 "85 per cent. 



Kesult. 



c.c. 



c.c. 



c.c. 





2 



o-i 



3-9 



Incomplete hemolysis 



2 



0-25 



3 75 





2 



0-5 



3 5 



)) ;) 





"01-per-cent. sol. 







2 



o-i 



3-9 





2 



0-25 



3 -75 



): j) 



2 



0-5 



3-5 



JJ )> 



Complete hcemolysis 



2 



1 



3 



2 



2 



2 



>> >> 



Incubated for three hours at 37° C. 



Thus 1 c.c. of a - 01-per-cent. solution of saponin in a 0'85-per-cent. 

 solution of NaCl completely hemolyses 2 c.c. of a 5-per-cent. blood suspension 

 in a total volume of 6 c.c. 



It was found, however, in the course of the experiments, that the least 

 amount of saponin needed to cause haemolysis varied slightly with different 

 specimens of rabbit's blood. For this reason it was necessary always to have 

 the serum of a rabbit fed on extracted bran alone to compare with the 

 serum of a rabbit fed on extracted bran and cholesterol, etc., so that the 

 comparative experiments were carried out on the corpuscles of the same 

 rabbit. 



Experiment II. — Eabbit 1 ; fed on extracted bran for 10 days.; weight of 

 rabbit without blood = 1*7 kilogrammes. 



Eabbit 2 ; fed for 10 days on extracted bran, during the last six days 

 given \ gramme cholesterol per day. Weight of rabbit without blood 

 = 2*5 kilogrammes. 



Eabbit 3 ; fed for 10 days on extracted bran, during the last six days 

 given \ gramme cholesterol per day for the first three days, 1 gramme 

 per day for the last three days. Total, 3f grammes cholesterol. 



Animals kept under the same conditions and killed at the same time. 

 Sera collected 36 hours after death. 



Eesults of experiment are given in the following tables : — 



