234 Miss Fraser and Mr. Gardner. Origin and [Apr. 3, 



Table II. — Inhibitory Action of Serum of Extracted Bran-fed Eabbit 



(Eabbit 1). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount of 

 serum. 



Amount of NaCl, 

 - 85 per cent. 



Amount of saponin, 

 "01 per cent. 



Eesult. 



c.c. 



CO. 



c.c. 







2 



0-025 



2-975 





Almost complete 











haemolysis 



2 



0-05 



2-95 



1 



Slight haemolysis 



2 



o-i 



2-9 . 



1 



Trace haemolysis 



2 



0-5 



2-5 



1 



No haemolysis 



2 



1 



2 



1 



>> 



Table III. — Inhibitory Action of Serum of Eabbit fed on Extracted Bran 



+ Cholesterol (Eabbit 2). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount of 

 serum. 



Amount of NaCl, 

 "85 per cent. 



Amount of saponin, 

 -01 per cent. 



Eesult. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2-975 



1 



No haemolysis 



2 



0-05 



2 -95 



1 



91 



2 



0-1 



2 -9 



1 



JJ 



2 



0-5 



2-5 



1 



1) 



2 



1 



2 



1 



JJ 



Table IV. — Inhibitory Effect of Serum of Eabbit fed on Extracted Bran 



+ Cholesterol (Eabbit 3). 



Amount of blood, 

 5-per-cent. 

 suspension. 



■ 



Amount of 

 serum. 



Amount of NaCl, 

 -85 per cent. 



Amount of saponin, 

 - 01 per cent. 



Eesult. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2-975 



1 



No haemolysis 



2 



-05 



2-95 



1 





2 



o-i 



2-9 



1 



5! 



2 



0-5 



2-5 



1 





2 



1 



2 



1 



» 



Incubated for three hours at 37° C. 



In order to control these figures, the experiments were repeated the 

 following day, with identical results. It was found, however, that when 

 kept over a period of several days the sera lost their inhibitory effect to 

 some extent, but even then the relative strengths remain in the same 

 proportion. 



