1909.] Destiny of Cholesterol in the Animal Organism. 235 



. Experiment III. — Eabbit 4 ; fed on extracted bran for 14 days, weight 

 = 1"5 kilogrammes. 



Eabbit 5 ; fed on extracted bran for 14 days, the last eight days with 

 cholesterol in addition ; \ gramme the two first, \ gramme the next four, 

 and 1 gramme the last two days. Total cholesterol, 4£ grammes ; weight 

 = 2 kilogrammes. 



Animals kept under similar conditions and killed at the same time. 

 Serum collected day after death. 



The results of experiments are tabulated below : — 



Table V. — Inhibitory Effect of Serum of Eabbit fed on Extracted Bran 



(Eabbit 4). 



Amount of blood, 

 5-per-eent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCI, 

 '85 per cent. 



Amount of saponin, 

 "01 per cent. 



Eesult. 



c.c. 



c.c. 



c.c' • 



c.c. 





2 



0-025 



2-975 



1 



Almost complete haemo- 



2 



0-05 



2-95 



1 



lysis 



Considerable haemolysis 



2 



o-i 



2-9 



1 



A trace haemolysis 



' ' 2 



0-5 



2-5 



1 



No haemolysis 



Table VI. — Inhibitory Effect of Serum of Eabbit fed on Extracted Bran 



+ Cholesterol (Eabbit 5). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCI, 

 -85 per cent. 



Amount of saponin, 

 -01 per cent. 



Eesult. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2-975 



1 



Considerable haemolysis 



2 



0-05 



2 -95 



1 . 



A. trace haemolysis 



2 



o-i 



2-9 



1 



No haemolysis 



2 



0-5 



2 -5 



1 '.. . 





Exactly similar results were given by the sera heated for over ah hour to 

 56° C, as the following table shows. The heating was performed with the 

 object of showing that the inhibitory action of the sera on haemolysis is not 

 due to an organic enzyme. 



