1909,] Destiny of Cholesterol in the Animal Organism. 237 



Table X. — Inhibitory Action of 10-per-cent. Solution of Serum of Babbit fed 

 on Extracted Bran + Cholesterol (Babbit 5). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum, 

 10 per cent. 



Amount of XaCl, 

 - 85 per cent. 



Amount of saponin, 

 - 01 per cent. 



Result. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



-25 



2 -75 



1 



Hsemolysed 



2 



0-5 



2-5 



1 



A trace hEeniolysis 



2 



1 -o 



2-0 



1 



Xo heemolysis 



2 



1-5 



1 5 



1 





We repeated these experiments with three other pairs of rabbits, and the 

 results were so similar that we need not recapitulate them. It is obvious, 

 therefore, that the cbolesterol absorbed from the food appeared in the blood 

 stream in the free state. Whether any entered in the form of esters it is 

 impossible to say, but experiments are in progress which w T e hope will throw 

 light on this subject. 



It now seemed of interest to ascertain whether, the animals being; fed on 

 esters of cholesterol in addition to extracted bran, the esters were absorbed 

 entirely as such, or whether they undergo hydrolysis in the process of 

 digestion, and find their way into the blood stream as free cholesterol, in 

 part, at any rate. 



For this purpose we made use of pure cholesterol oleate and cholesterol 

 stearate for feeding the animals. 



Experiment IV. — Babbit 6 ; fed on extracted bran for 18 days ; weighed 

 2 - 2 kilogrammes at beginning, 2 - 3 kilogrammes at end of experiment. 



Babbit 7 ; fed on extracted bran for eight days, the last six days fed with 

 cholesterol oleate in addition ; £ gramme for four days, 1 gramme per day 

 for two days : weight of animal = 2 kilogrammes at beginning and end of 

 feeding. 



Babbit 8 ; fed on extracted bran for eight days, the last six days fed with 

 cholesterol stearate in addition ; \ gramme for four days, 1 gramme per day 

 for two days ; weight of animal = 2 kilogrammes at beginning and end of 

 feeding. 



Animals were kept under the same conditions and killed at the same time. 

 Serum collected next day. 



Results of experiment are tabulated below : — 



