240 Miss Fraser and Mr. Gardner. Origin and [Apr. 3, 



Table XVII. — Inhibitory Action of Serum in 10-per-cent. Solution. 

 Extracted Bran-fed Eabbit (Eabbit 6). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum, 

 10 per cent. 



Amount of NaCl, 

 "85 per cent. 



Amount of saponin, 

 - 01 per cent. 



Result. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-25 



2 5 



1 -25 



Almost complete haemo- 











lysis 



2 



0-5 



2 25 



1-25 



Considerable haemolysis 



2 



1 -o 



1-75 



1 -25 



Slight haemolysis 



2 



2-5 



0-25 



1 -25 



Table XVIII.— Inhibitory Action of Serum of Eabbit fed on Extracted Bran 

 + Cholesterol Oleate in 10-per-cent. Solution (Eabbit 7). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCl, 

 "85 per cent. 



Amount of saponin, 

 "01 per cent. 



Eesult. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-25 



2-5 



1 -25 



Haemolysis 



2 



0-5 



2 25 



1 '25 



Slight haemolysis 



2 



1 -o 



1-75 



1 -25 



2 



2-5 



0-25 



1 -25 



No haemolysis 



Table XIX. — Inhibitory Action of Serum of Eabbit fed on Extracted Bran 

 + Cholesterol Stearate in 10-per-cent. Solution (Eabbit 8). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum, 

 10 per cent. 



Amount of NaCl, 

 "85 per cent. 



Amount of saponin, 

 '01 per cent. 



Eesult. 



c.c. 



c.c. , 



c.c. 



c.c. 





2 



0-25 



2 -5 



1 -25 



Haemolysis 



2 



0-5 



2 -25 



1 -25 



Slight haemolysis 



2 



1-0 



1-75 



1 "25 



2 



2 -5 



0-25 



1 "25 



No haemolysis 



These experiments clearly show that the esters of cholesterol undergo 

 hydrolysis during the digestive process and appear, partially at any rate, in 

 the blood stream as free cholesterol. 



As cholesterol is not a normal constituent of the food of rabbits, it 

 appeared interesting to find out (1) whether vegetable phytosterol is 

 absorbed by the animal if given in the food ; (2) whether this can be- 

 utilised by the animal. 



Experiment V. — To ascertain whether the phytosterol of the food in the 



