242 Miss Fraser and Mr. Gardner. Origin and [Apr. 3, 



For this purpose animals were fed with actual bran and wheat-germ, which 

 is rich in phytosterol, and other animals were fed on extracted bran with the 

 addition of measured quantities of pure phytosterol. 



Kabbit 9 ; fed on extracted bran for 10 days ; weight of animal 

 = 1-4 kilogrammes. 



Kabbit 10 ; fed on extracted bran for three days, then ordinary bran and 

 wheat-germ for six clays ; weight of animal = 1*7 kilogrammes. The 

 following table gives the result of experiment : — 



Table XX. — Inhibitory Effect of Serum of Eabbit fed on Extracted Bran 



(Eabbit 9). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCl, 

 "85 per cent. 



Amount of saponin, 

 '01 per cent. 



Eesult. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2 975 



1 



Almost complete hsemo- 











lysis 



2 



0-05 



2-95 



1 



Slight haemolysis 



2 



o-i 



2 9 



1 



A trace haemolysis 



2 



0-5 



2-5 



1 



No haemolysis 



Table XXI. — Inhibitory Effect of Serum of Eabbit fed on Ordinary Bran and 



Wheat-germ (Eabbit 10). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCl, 

 "85 per cent. 



Amount of saponin, 

 "01 per cent. 



Eesult. 



. • , c.c. 



c.c. 



c.c. 



: . - c.c. > , ■ 





2 



0-025 



2-975 



1 



Haemolysis 



2 



0-05 



2 -95 



1 



Slight haemolysis 



2 



o-i 



2-9 



1 



No haemolysis 



2 



0-5 



2-5 



1 



» 



This experiment was repeated the following day with identical results. 

 Eabbit 11 ; fed on extracted bran for 11 days ; weight of animal 

 = 2 kilogrammes. 



Eabbit 12 ; fed on ordinary bran and wheat-germ for nine days, having 

 been fed with extracted bran for two days previously ; weight of animal 

 = 2 kilogrammes. 



The animals were kept under exactly similar conditions and killed at the 

 same time. Serum collected day after death. The results are tabulated 

 below : — 



