1909.] Destiny of Cholesterol in the Animal Organism. 243 



Table XXII. — Inhibitory Action of Serum of Eabbit fed on Extracted Bran 



(Rabbit 11). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCI, 

 - 85 per cent. 



Amount of saponin. 

 -01 per cent. 



Result. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2-975 



1 



Almost complete haemo- 











lysis 



2 



0-05 



2-95 



1 



Haemolysis 



2 



-1 



2-9 



1 



Slight haemolysis 



2 



0-5 



2-5 



1 



No haemolysis 



Table XXIII. — Inhibitory Action of Serum of Eabbit fed on Ordinary Bran 

 and Wheat-germ (Babbit 12). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCI, 

 "85 per cent. 



Amount of saponin, 

 "01 per cent. 



Result. 



' c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2-975 



1 



Haemolysis 



2 



-05 



2-95 



1 



Slight haemolysis 



2 



o-i 



2-9 



1 



A trace haemolysis 



2 



0-5 



2-5 



1 



No haemolysis 



Experiments were also carried out with the sera after heating for over an 

 hour to 55° C, and in 10-per-cent. solutions made up with physiological salt. 

 The results agreed entirely with the above tables. 



The serum of the rabbits fed on ordinary bran and wheat-germ shows 

 a slightly greater inhibitory power than the serum of the rabbit fed on 

 extracted bran. This seems to indicate (1) that some of the phytosterol of 

 the wheat-germ found its way into the blood stream, or (2) possibly caused 

 an increase of cholesterol in the blood. Babbits experimented on disliked 

 the wheat-germ so that it was often neglected, the extracted bran being 

 always given the preference. We therefore resolved to give, in subsequent 

 experiments, weighed quantities of pure phytosterol. 



Babbit 13 ; fed on extracted bran for seven days. 



Babbit 14 ; fed on extracted bran for 12 days with phytosterol in addition, 

 eight days with f gramme per day, then four days with \ gramme per day. 

 Animals kept under similar conditions. 



