244 Miss Fraser and Mr. Gardner. Origin and [Apr. 3, 



Table XXIV. — Inhibitory Action of Serum of Eabbit fed with Extracted 



Bran (Eabbit 13). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCl, 

 "85 per cent. 



Amount of saponin, 

 '01 per cent. 



Result. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2 -975 



1 



Complete haemolysis 



2 



0-05 



2-95 



1 



Considerable haemolysis 



2 



o-i 



2-9 



1 



Slight haemolysis 



2 



0-5 



2-5 



1 



No haemolysis 



Table XXV. — Inhibitory Action of Serum of Eabbit fed with Extracted Bran 



+ Phytosterol (Eabbit 14). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCl, 

 -85 per cent. 



Amount of saponin, 

 -01 per cent. 



Result. 



c.c. 



c.c. 



c.c. 



c.c. 





2 



0-025 



2-975 



1 



Haemolysis not complete 



2 



0-05 



2-95 



1 



A trace haemolysis 



2 



o-i 



2-9 



1 



No haemolysis 



2 



0-5 



2 -5 



1 





These experiments were repeated with the same sera, on consecutive days, 

 three times altogether, and in every case the result was identical. 



Further, it was found that on addition of greater quantities of saponin to 

 the same volume of blood, if the serum were added in proportional 

 quantities, haemolysis was in every case prevented, if the ratio between 

 saponin and serum were the same as that which prevented haemolysis in the 

 above experiment. 



The table is given below : — 



Table XXVI. — Inhibitory Effect of Serum of Eabbit fed on Extracted Bran 

 with Phytosterol in addition (Eabbit 14). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCl, 

 -85 per cent. 



Amount of saponin, 

 - 01 per cent. 



Result. 



c.c. . 



c.c. 



c.c. 



c.c. 





2 



0-025 



2-975 



1 



Haemolysis 



2 



0-05 



2 -95 



1 



A trace haemolysis 



2 



o-i 



2 -9 



1 



No haemolysis 



2 



0-15 



2-35 



1 -5 





2 



0-2 



1-8 



2 





2 



0-25 



1 -25 



2-5 





