1909i] Destiny of Cholesterol in the Animal Organism. 245 



This is interesting as giving an indication of the quantitative reaction 

 between the serum and saponin, and therefore of its chemical nature. 



In order to ascertain whether the result would be affected by heating or 

 dilution, however, another pair of rabbits was used. 



Eabbit 15 ; fed for 14 days on extracted ' bran. Weight of animal 

 = 1*5 kilogrammes. 



Eabbit 16 ; fed on ordinary bran and wheat-germ for 13 days, the last 

 eight days with phytosterol in addition ; for six days \ gramme per day, then 

 ■f gramme the next day, and 1 gramme the last day.: 



Animals kept under similar conditions and killed! at the same time. The 

 results of experiments are given below : — 



Table XXVII. — Inhibitory Effect of Serum of Eabbit fed on Extracted Bran 



(Eabbit 15). 



Amount of blood, 

 5-per-cent. 

 suspension. 



Amount 

 of serum. 



Amount of NaCl, 

 -85 per cent. 



Amount of saponin, 

 -01 per cent. 



Besulfc. 













c.c. 

 2 



S 

 2 

 2 



c.c. 

 0-025 



0-05 



0-1 



0-5 



c.c. 

 2-b75 



2-95 

 2-9 

 2 -5 



c.c. 

 1 



1 

 1 

 1 



Haemolysis hot quite 



complete 

 Considerable haemolysis 

 Slight haemolysis 

 No haemolysis 



Table XXVIIL— Inhibitory Effect of Serum of Eabbit fed with Phytosterol 

 in addition to Ordinary Bran and Wheat-germ (Eabbit 16). 



Amount of blood, 

 5-per-cent. 

 suspension. 



■ 



Amount 

 of serum. 



Amount of NaCl, 

 -85 per cent. 



Amount of saponin, 

 - 01 per cent. 



Result; 



- 











c.c: 

 2 

 2 

 2 

 2 



c.c. 

 0-025 

 0-05 



o-i 



0-5 



c.c. 

 2-975 

 2 -95 

 2-9 

 2 -5 



c.c. 

 1 

 1 

 1 

 1 



Haemolysed 



A trace of haemolysis 



No haemolysis 



VOL. LXXXI. — B. 



S 



