332 Dr. R T. Hewlett. The Effect of the Injection of [June 5, 



produced an alteration (negative phase and subsequent rise) less marked than 

 with the endotoxin prepared from the unextracted bacilli (Eabbit XIII). 



Index 

 2-0 

 I -9 

 1-8 

 1-7 

 1-6 



1-5 

 1-4 

 1-3 

 1-2 

 1-1 

 I -0 

 -9 

 -8 

 • 7 

 0-6 

 ' 5 

 4 



RABBIT X 



RABBIT XI 



RABBIT XII 



4 \ 



RABBIT XIII 



^ T co > 



t O J" > X 



Chart V. — Tuberculin and Tubercle Endotoxin. 



Eabbit X received 0'002 milligramme Tuberculin E. 



Eabbit XI received 0"002 milligramme tubercle endotoxin. 



Eabbit XII received TO milligramme tubercle endotoxin. 



Eabbit XIII received TO milligramme ether-extracted tubercle endotoxin. 



D. Effect of keeping on the Activity of the Endotoxin Solutions. — In view 

 of the possible use of endotoxin solutions for vaccine treatment, it was 

 thought desirable to make tests on their activity after they had been kept 

 for a period. The tubercle endotoxin was prepared on March 9, 1908, and 

 the staphylococcus endotoxin was prepared on March 10, 1908. They were 

 the same solutions as those employed in the experiments detailed in 

 Sections B and C above, were kept in an ice-safe and were injected on 

 May 1, 1908, into fresh rabbits, i.e. approximately seven weeks after pre- 

 paration. The results are given graphically in Charts VI and VII of the 

 opsonic indexes. 



In the case of the tubercle endotoxin, doses similar to those given in the 

 experiments in Section C were administered. It will be seen from Chart VI 

 of the opsonic indexes that the endotoxin solutions were quite as active as 

 previously. Eabbit XV, receiving the large dose of endotoxin prepared from 

 unextracted bacilli, became unwell on the twelfth day, and died on the 

 thirty-sixth day, after injection. 



