1909.J 



Intracellular Constituents of Bacteria, etc. 



333 



Indej 

 2-4 



2 

 2 

 2 

 2 

 .1 

 1 

 1 

 1 

 1 

 1 

 1 

 1 



1 ■ 1 

 l'O 

 0-9 

 0-8 

 ft- 7 



3 



2 . 

 I 





 9 

 8 



7 . 

 6 

 5 

 4 



3 

 2 



RABBIT XIV 



•a « 



RABBIT XV 



\ ; 



> £ 



RABBIT XVI 



u » ^ « » • 



= > * * 

 > X o 



Chart VI. — Old Tubercle Endotoxin Solutions (7 weeks old). 



Babbit XIV received 0'002 milligramme tubercle endotoxin. 



Rabbit XV received l'O milligramme tubercle endotoxin. 



Rabbit XVI received l'O milligramme ether-extracted tubercle endotoxin. 



In the case of the staphylococcus endotoxin, in view of possible 

 deterioration on account of keeping, 10 times the dose (1 milligramme) 

 previously given was injected. It will be seen from Chart VII of the opsonic 

 indexes that a marked effect on the opsonic index was induced, greater, as 

 before, in the case of the endotoxin prepared from the recently isolated 

 strain (Eabbit XVIII). 



Another experiment with the latter endotoxin (prepared on March 9, 1908) 

 was performed on November 3, 1908, approximately eight months after 

 preparation, the dose being 01 milligramme. Again it will be seen from 

 Chart VIII of the opsonic indexes that a marked effect was produced 

 (Rabbit XIX). The staphylococcus endotoxin employed in the experiment 

 detailed in Section E below was the same preparation and was then 

 10 months old, and from Chart IX it will be seen that it was still very 

 active. 



These experiments indicate that the endotoxin solutions deteriorate but 

 slowly, and retain a considerable proportion of their activity for at least 

 three to six months. 



E. Production of " Negative Phase " by Injection of Endotoxin. — It was 



