1909.] The Alcoholic Ferment of Yeast-juice. 341 



Table VII. — Maxima attained by the Fermentation of Fructose in presence 



of Phosphate. 



Number 

 of 



experiment. 



Volume of '0"6 molar 

 phosphate solution 

 added. 



Total 

 Tolume. 



Maximum rate 

 attained, cubic 

 centimetres C0 2 in 

 5 minutes. 





c.c. 



c.c. 





13 



12 -o 



75 



80 



14 



12 -5 



50 



27-1 



15 



10 



50 



31 -2 



16 



20 



55 



76 -2 



(b) Equivalence of the extra carbon dioxide evolved to the phosphate 

 added. One example of this has already been given in Experiment 10, 

 p. 338. 



Experiment 17. — In another case the phosphate added was equivalent to 

 65-2 c.c. of C0 2 at N.T.P. 





c.c. 

 82 -2 

 10 -8 



Correction for rate of juice 1 - 2 c.c. per 

 5 minutes for a period of 45 minutes 



71 -4 





64 -2 





(c) Production of a hexosephosphate non-precipitable by magnesium 

 citrate mixture. 



Experiment 18. — The experiment was carried out precisely as Experiment 9. 

 The amounts of phosphate are expressed as Mg 2 P20 7 : — 









•3263 









•0426 









•2837 



The solution after boiling was found to contain a hexosephosphate which 

 has been isolated in the form of a lead salt and is at present undergoing 

 investigation. 



(d) Existence of an optimum concentration of phosphate. 

 The following table shows the maximum rates, produced by the addition 



2 c 2 



