344 Messrs. A. Harden and W. J. Young. [June 10, 



Time after addition. 



CO; evolved in preceding 5 minutes. 



Substances added. 



A. 



Glucose and phosphate. 



B. 



Fructose and phosphate. 



5 minutes 



1-5 



2-6 



10 „ 



1-8 



6-9 



15 „ 



1-2 



13 8 



!? 



1-5 



19-4 



25 „ 



1 -5 



25 -1 



17°'2 and 762 - 4 mm. being equivalent to 2 c.c. of the -phosphate solution. 

 The further course of the experiment was as follows : — 



B. 



Time after addition 

 of phosphate. 



Gas evolved since 

 last addition of 

 phosphate. 



Phosphate added 

 '6 molar solution. 



Rate per 

 5 minutes. 



minutes. 



c.c. 



c.c. 



c.c. 



25 



67 -8 





25 -1 



30 







27 -1 



35 



53 9 





26 -8 



40 







22-1 



45 







19 -7 



50 







18 -5 



55 







19 -8 



60 







20 5 



65 



120 -5 



10 



19 -9 



70 







17 3 



75 







18 -2 



80 







17 -4 



85 







15 -8 



90 







16 -8 



95 



97 -9 





12 -4 



Total gas evolved 



340-1 





Hence, although the concentration of the phosphate was never allowed to 

 fall much below the original value and was generally considerably above it, 

 the addition of - 2 gramme of fructose to 2 grammes of glucose produced 

 a total evolution of 340 - l c.c. of CO2, corresponding to the total fermentation 

 of 1*3 grammes of sugar (in the ratio C 6 Hi20 6 : 2 C0 2 ), whereas in the 

 absence of fructose the total fermentation would have been only 28 - 5 c.c, 

 corresponding to 0-22 gramme of sugar. As the fructose added was only 

 0*2 gramme and the subsequent evolution of carbon dioxide corresponded to 



