350 The Discovery of a Remedy for Malignant Jaundice, etc. 



a while, all parasites are lost to view. When, after an interval, the parasites 

 reappear, they do so in exceedingly small numbers, and, after a while, they 

 disappear completely and finally. 



All of our dogs were treated by subcutaneous injections of saturated 

 solutions of the dyes. 



Bovine Piroplasinosis. 



With regard to redwater, we are in a position to state that trypanblau 

 exerts a very prompt effect upon the parasite. The effect is precisely 

 similar to that observed in Piroplasma canis. Our experiments upon the 

 bovine disease are still in progress, but we feel that they are sufficiently 

 advanced to warrant the trial of the remedy in the field. We shall report 

 upon our results in a future communication. 



The results of these experiments are of considerable interest, since they 

 throw additional light upon the biology of the parasites and entirely confirm 

 the observations of Nuttall and Graham-Smith upon the usual mode of 

 multiplication of the parasites in the circulating blood. The striking effects 

 of the drugs upon the parasites led us directly to make enumerations of the 

 different forms of parasites occurring in the blood of treated and untreated 

 animals. The result of these observations has been to bring to light several 

 interesting facts regarding the life-history of the parasites. 



East Coast Fever. 



Incidentally we may mention that in one experiment which we have tried, 

 trypanblau exerted no effect whatsoever on the parasite of East Coast Fever 

 in cattle. This does not appear to us surprising, since the parasite is very 

 different from Piroplasma, although most writers still persist in retaining it 

 in the genus. For reasons stated elsewhere by Nuttall (1908), the parasite 

 of East Coast Fever should be named Theileria parva. 



Conclusions. 



The obvious practical conclusions to be drawn from our results is that the 

 remedies will prove of value in practice. It is highly probable that they will 

 act in a similar manner in relation to equine and ovine piroplasmosis. The 

 mere fact that remedial agents have been found for diseases which have 

 hitherto run their course, in spite of all treatment, is encouraging, since with 

 time we may reasonably hope to cope with these maladies in an efficient 

 manner. 



