1909.] 



Trypanosoma gambieuse in Glossina palpalis. 



411 



Description of the Glossina palpalis, Fly 866, which was dissected 

 75 Days after having fed on a Monkey whose Blood contained 

 Trypanosoma gambiense. 



Experiment 866. 



May 19. 1909. — Dissected a Glossina palpalis, which was found dead to-day 

 in Cage A of Experiment 663. On removing the viscera by the usual 

 method, the mid-gut was seen to be of a pale salmon-pink. A small 

 quantity of its contents, examined in the fresh condition, was found 

 to contain enormous numbers of trypanosomes. The tube of this part of the 

 intestine was absolutely crammed with active, seething masses of these 

 flagellates. In regard to the other parts of the flyj nothing was seen in the 

 proboscis. In the proventriculus one trypanosome only was found. The 

 salivary glands contained large numbers of altered-looking trypanosomes, 

 the fore-gut many large stout forms, with bright granules. The crop was 

 empty and showed nothing. The Malpighian tubules, hind-gut, and 

 proctodseum also were drawn blank. 



In addition to examining these organs in the fresh condition, smears were 

 made and stained. The examination of these stained specimens gave the 

 following results : — 



The salivary glands. — These had been carefully removed before the intestine 

 was opened, and therefore had no chance of being fouled. As will be seen from 

 the coloured drawing (Plate 10, fig. 1), the trypanosomes found in these glands 

 differed from those seen in the -intestine. The bodies are very irregular in 

 shape, and contain, besides a reddish-stained nucleus, dark deeply-stained coarse 

 chromatin granules. The other cell contents remain unstained. Free 

 chromatin granules and flagella are to be seen scattered over the field. 

 Sometimes the bodies are definitely pear-shaped, with a fiagellum coming 

 from the narrow end, and rarely a more definite trypanosome shape can b 

 seen ; but never a true trypanosome. 



[It is a matter of deep regret that an inoculation experiment was not made 

 with an emulsion of part of the salivary glands.] 



The fore-gut. — The fore-gut contained many trypanosomes. The cytoplasm 

 stains a pale blue, and the nucleus a reddish-purple. The micronucleus is 

 not distinctly seen in some of the trypanosomes, but when it is, it is 

 always distinctly posterior to the nucleus. The protoplasm contains many 

 coarse darkly-stained chromatin granules. The undulating membrane is 

 less marked than in the normal blood trypanosome, and the fiagellum, which 

 usually springs from a micronucleus-like body, is less deeply stained 

 (Plate 11, figs. 6—13). 



