414 



Col. Sir D. Bruce and others. On a 



[July 5, 



DESCRIPTION OF PLATES. 

 Plate 10. 



Smear preparation of salivary glands of Olossina palpalis, Experiment 866, stained 

 Giemsa, showing irregularly shaped trypanosomes, with unstained protoplasm, reddish- 

 coloured nuclei, and deeply stained chromatin granules. Note the chromatin granules 

 scattered singly about the field, each surrounded by a pale area, fig. 1. x 2000. 



Normal Trypanosoma gambiense from monkey, Experiment 568, on which the flies were 

 fed at the beginning of the experiment, figs. 2, 3, 4, and 5. x 2000. 



Trypanosomes from the mid-gut of infected fly, Experiment 866, figs. 6 — 16. x 2000. 



Plate 11. 



Eosette form from the mid-gut, fig. 1. x 2000. 



Trypanosoma gambiense from the blood of monkey, Experiment 868, into which a tiny 

 drop of the contents of the mid-gut of Fly 866 had been injected, figs. 2 — 5. x 2000. 

 Trypanosomes from the fore-gut of Fly 866, stained Giemsa, figs. 6 — 13. x 2000. 



A Note on the Occurrence of a Trypanosome in the African 



Elephant. 



By Colonel Sir David Bruce, C.B., F.B.S., Army Medical Service ; Captains 

 A. E. Hamerton, D.S.O., and H. E. Bateman, Boyal Army Medical 

 Corps ; and Captain E. P. Mackie, Indian Medical Service. (Sleeping 

 Sickness Commission of the Boyal Society, 1908.) 



(Eeceived July 5, 1909.) 



[Plate 12.] 



As trypanosomes have never been reported as having been observed in 

 the blood of the African Elephant, the Commission thought it would be of 

 interest to note this observation. 



In Laveran and Mesnil's book on trypanosomes, translated by Nabarro, 

 on p. 261 it is stated that " the occurrence of Surra {Trypanosoma evansi) in 

 elephants in India and Burmah is practically proved. In this connection we 

 have only the statement of G. H. Evans that, in 1893, 14 out of 

 32 elephants died of the disease in Burmah." The year 1893 is almost 

 prehistoric for trypanosomes. At that time observers had even failed 

 to distinguish between the common rat trypanosome — Trypanosoma levjisi — 

 and that of Surra. It may well be, then, that Evans was mistaken in his 

 diagnosis of the species causing this large mortality in elephants. 



The African elephant, in whose blood this trypanosome was found, was 



