1909.] Trypanosoma in the African Elephant. 415 



shot by Mr. L. C. Lea- Wilson, of the Uganda Company, Limited, at a spot 

 two miles from the eastern shore of Lake Albert, near Ngogole, about 

 31° 10' E. lat. and 1° 30' K long. It is to be regretted that none of the 

 blood was injected into a dog, donkey, or ox, in order that a fuller study of 

 this trypanosome might have been made. As it is, all the material 

 available are a couple of smears made by Mr. Lea- Wilson and sent to the 

 Commission. 



Morphology of the Trypanosome of the Elephant. 



Method of Fixing and Staining. — The two slides received from Mr. Lea- 

 Wilson were fixed in osmic acid vapour and alcohol, stained in Giemsa, and 

 decolorised in orange tannin.* 



Length. — For method of measurement see the same paper, p. 16. As will be 

 seen from the coloured plate, which was drawn by Sergeant Gibbons, 

 E.A.M.C., this trypanosome is of medium size. The average length of 

 18 individuals is 18*5 microns : maximum 21, minimum 15. 



Breadth. — On an average the breadth at the thickest part is 3 microns. 



Shape. — This trypanosome is of the Trypanosoma hrucei type, inasmuch as 

 it has a well-developed undulatory membrane and free flagellum. As will 

 be seen from the drawing (Plate 12), one noteworthy feature it has is the 

 uniformity in size and shape of the different individuals. The posterior end 

 is blunt, or conical, reminding one somewhat of the head of a seal, with the 

 bulging micronucleus for an eye. The body thickens as far as the middle, 

 when it gradually tapers away to the anterior end. 



Contents of Cell. — The protoplasm is clear and particularly free from 

 granules. 



Nucleus. — The nucleus is compact and sharply defined from the neigh- 

 bouring protoplasm. In shape it is round, or oval, and often lies nearer the 

 anterior extremity than the posterior. Its length averages 2 microns. 



Micronucleus. — The micronucleus is small, round and distinct. It is 

 situated close to the posterior extremity, and often appears to bulge above 

 the surface. 



Undulating Membrane. — The undulating membrane is well developed and 

 thrown into well-marked folds. 



Flagellum. — The flagellum stains deeply. It runs from the micronucleus 

 along the edge of the undulating membrane, beyond which it projects 

 as a free flagellum for some 5 or 6 microns. 



* Vide ' Eoy. Soc. Proc.,' Series B, vol. 81, p. 16. 



